Abstract | PURPOSE: To evaluate the comparative effect of topical versus intramuscular administration of nanoparticle-carried DNA vaccine in preventing corneal herpes simplex virus type 1 (HSV-1) infection. METHODS: Nanoparticle [ polyethylenimine (PEI)-Fe3O4]-carried DNA vaccine (PEI-Fe3O4-pRSC-gD-IL-21) or DNA vaccine (pRSC-gD-IL-21) alone were topically versus intramuscularly inoculated into one eye each of mice on days 0, 14 and 28. Three weeks after the final immunization, the specific immune responses and clinical degrees of primary herpes simplex keratitis were evaluated. RESULTS: Topical inoculation of nanoparticle-carried DNA vaccine induced mice to generate similar levels of specific HSV-1-neutralizing antibody, IFN-γ and IL-4 in serum and specific killing (cytotoxicity) and proliferative activities of the splenic lymphocytes, but a significantly higher level of secretory IgA in tears compared to those of intramuscular inoculation. More importantly, the mice inoculated topically showed a significantly decreased herpes simplex keratitis severity than the mice inoculated intramuscularly after HSV-1 challenge on the corneas of the mice. CONCLUSIONS:
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Authors | Kai Hu, Tejsu Malla, Yujia Zhai, Lili Dong, Ru Tang |
Journal | Ophthalmic research
(Ophthalmic Res)
Vol. 55
Issue 2
Pg. 99-110
( 2015)
ISSN: 1423-0259 [Electronic] Switzerland |
PMID | 26650397
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 S. Karger AG, Basel. |
Chemical References |
- Antibodies, Neutralizing
- Antibodies, Viral
- Cytokines
- Drug Carriers
- Immunoglobulin A, Secretory
- Interleukins
- Vaccines, DNA
- Viral Envelope Proteins
- glycoprotein D, Human herpesvirus 1
- interleukin-21
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Topics |
- Administration, Topical
- Animals
- Antibodies, Neutralizing
(blood)
- Antibodies, Viral
(blood)
- Cytokines
(blood)
- Disease Models, Animal
- Drug Carriers
- Fluorescent Antibody Technique, Indirect
- Herpesvirus 1, Human
(genetics, immunology)
- Immunity, Cellular
- Immunization
(methods)
- Immunoglobulin A, Secretory
(blood)
- Injections, Intramuscular
- Interleukins
(genetics)
- Keratitis, Herpetic
(immunology, prevention & control)
- Male
- Mice
- Mice, Inbred BALB C
- Microscopy, Confocal
- Nanoparticles
- Real-Time Polymerase Chain Reaction
- Vaccination
(methods)
- Vaccines, DNA
(administration & dosage)
- Viral Envelope Proteins
(genetics)
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