Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing.

Abstract

The purpose of this study was to permit bone marrow mesenchymal stem cells (BMSCs) to reach their full potential in the treatment of chronic wounds. A biocompatible multifunctional crosslinker based temperature sensitive hydrogel was developed to deliver BMSCs, which improve the chronic inflammation microenvironments of wounds. A detailed in vitro investigation found that the hydrogel is suitable for BMSC encapsulation and can promote BMSC secretion of TGF-β1 and bFGF. In vivo, full-thickness skin defects were made on the backs of db/db mice to mimic diabetic ulcers. It was revealed that the hydrogel can inhibit pro-inflammatory M1 macrophage expression. After hydrogel association with BMSCs treated the wound, significantly greater wound contraction was observed in the hydrogel + BMSCs group. Histology and immunohistochemistry results confirmed that this treatment contributed to the rapid healing of diabetic skin wounds by promoting granulation tissue formation, angiogenesis, extracellular matrix secretion, wound contraction, and re-epithelialization. These results show that a hydrogel laden with BMSCs may be a promising therapeutic strategy for the management of diabetic ulcers.

• Authors: Shixuan Chen, Junbin Shi, Min Zhang, Yinghua Chen, Xueer Wang, Lei Zhang, Zhihui Tian, Yuan Yan, Qinglin Li, Wen Zhong, Malcolm Xing, Lu Zhang, Lin Zhang
• Journal: Scientific reports (Sci Rep) Vol. 5 Pg. 18104 (Dec 08 2015) ISSN: 2045-2322 [Electronic] England
• PMID: 26643550 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biocompatible Materials
• Hydrogel, Polyethylene Glycol Dimethacrylate

Topics

• Animals
• Biocompatible Materials (chemistry, therapeutic use)
• Diabetes Complications (pathology, therapy)
• Disease Models, Animal
• Granulation Tissue (metabolism, pathology)
• Hydrogel, Polyethylene Glycol Dimethacrylate (chemical synthesis, chemistry)
• Male
• Materials Testing
• Mesenchymal Stem Cell Transplantation (methods)
• Mesenchymal Stem Cells (metabolism)
• Mice
• Skin Ulcer (etiology, pathology, therapy)
• Wound Healing