Development of a Synthetic Surfactant Using a Surfactant Protein-C Peptide Analog: In Vitro Studies of Surface Physical Properties.

Abstract

PURPOSE:

Pulmonary surfactant (PS) replacement has been the gold standard therapy for neonatal respiratory distress syndrome; however, almost all commercial PSs contain animal proteins. We prepared a synthetic PS by using a human surfactant protein (SP) analog and evaluated its in vitro properties.

MATERIALS AND METHODS:

A peptide sequence (CPVHLKRLLLLLLLLLLLLLLLL) of human SP-C was chosen to develop the peptide analog (SPa-C). The new synthetic SP-C PS (sSP-C PS) was synthesized from SPa-C, dipalmitoyl phosphatidylcholine, phosphatidyl glycerol, and palmitic acid. Physical properties of the sSP-C PS were evaluated by measuring the maximum and minimum surface tensions (STs), surfactant spreading, and adsorption rate. In addition, we recorded an ST-area diagram. The data obtained on sSP-C PS were subsequently compared with those of purified natural bovine surfactant (PNBS), and the commercial product, Surfacten®.

RESULTS:

The sSP-C PS and Surfacten® were found to have maximum ST values of 32-33 mN/m, whereas that of PNBS was much lower at 19 mN/m. The minimum ST values of all three products were less than 10 mN/m. The values that were measured for the equilibrium ST of rapidly spreading sSP-C PS, Surfacten®, and PNBS were 27, 27, and 24 mN/m, respectively. The surface adsorptions were found to be the same for all three PSs (20 mN/m). ST-area diagrams of sSP-C PS and Surfacten® revealed similar properties.

CONCLUSION:

In an in vitro experiment, the physical properties exhibited by sSP-C PS were similar to those of Surfacten®. Further study is required to evaluate the in vivo efficacy.

• Authors: Chong Woo Bae, Sung Hoon Chung, Yong Sung Choi
• Journal: Yonsei medical journal (Yonsei Med J) Vol. 57 Issue 1 Pg. 203-8 (Jan 2016) ISSN: 1976-2437 [Electronic] Korea (South)
• PMID: 26632402 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• C-Peptide
• Pulmonary Surfactant-Associated Protein C
• Pulmonary Surfactants
• Surface-Active Agents
• 1,2-Dipalmitoylphosphatidylcholine
• colfosceril palmitate

Topics

• 1,2-Dipalmitoylphosphatidylcholine (analogs & derivatives)
• Adsorption
• Amino Acid Sequence (genetics)
• Animals
• C-Peptide (chemistry)
• Cattle
• Humans
• Infant, Newborn
• Pulmonary Surfactant-Associated Protein C (chemical synthesis, pharmacology)
• Pulmonary Surfactants (chemical synthesis, pharmacology)
• Respiratory Distress Syndrome, Newborn (drug therapy)
• Surface Properties
• Surface Tension
• Surface-Active Agents