Generation of Viable Mice from Induced Pluripotent Stem Cells (iPSCs) Through Tetraploid Complementation.

Abstract

Tetraploid complementation assay is the most rigorous criteria for pluripotency characterization of pluripotent stem cells including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Pluripotent stem cells could complement the developmental deficiency of tetraploid embryos and thus support the full-term mice development. Here we describe the protocol for tetraploid complementation using iPSCs to produce viable all-iPSC mice.

• Authors: Lan Kang, Shaorong Gao
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 1330 Pg. 125-32 ( 2015) ISSN: 1940-6029 [Electronic] United States
• PMID: 26621595 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Blastocyst (cytology, metabolism)
• Cell Culture Techniques
• Cell Differentiation
• Cloning, Organism
• Embryo, Mammalian
• Embryonic Development
• Induced Pluripotent Stem Cells (cytology, metabolism)
• Mice
• Microinjections
• Tetraploidy