Thymic Stromal Lymphopoietin Induction Is Mediated by the Major Whey Proteins α-Lactalbumin and β-Lactoglobulin through the NF-κB Pathway in Immune Cells.

Abstract

α-Lactalbumin and β-lactoglobulin are two major whey proteins that specifically bind immunoglobulin E and are suspected as major allergens causing cow's milk allergy (CMA). Recent studies have shown that thymic stromal lymphopoietin is a critical factor linking at the interface of the body and environment to the T-helper 2 response. However, it is not known whether thymic stromal lymphopoietin expression is changed by α-lactalbumin and β-lactoglobulin in immune cells. Using RT-PCR and ELISA, the present study was conducted to examine if intravenous injection of α-lactalbumin and β-lactoglobulin increased pro-inflammatory cytokines, T-helper 2 cytokines, and thymic stromal lymphopoietin expression in several immune cells, including macrophages, mast cells, and keratinocytes. Results showed that α-lactalbumin and β-lactoglobulin induced thymic stromal lymphopoietin, interleukin-6, and tumor necrosis factor-α expression. It was concluded that the allergenicity of α-lactalbumin and β-lactoglobulin may be attributed to thymic stromal lymphopoietin induction, T-helper 2 cytokines, and pro-inflammatory cytokines.

• Authors: Jae-Min Yoo, Young W Park, Sun Young Yoon, Ji Yoon Son, Seok Geun Jeong, Beom-Young Park, Jae Wha Kim, Myoung Soo Nam
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 63 Issue 50 Pg. 10803-10 (Dec 23 2015) ISSN: 1520-5118 [Electronic] United States
• PMID: 26618482 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• Cytokines
• Interleukin-6
• Lactoglobulins
• NF-kappa B
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• Lactalbumin
• Thymic Stromal Lymphopoietin

Topics

• Allergens (immunology)
• Animals
• Cattle
• Cell Line
• Cytokines (analysis, blood, genetics, immunology, physiology)
• Gene Expression
• Humans
• Interleukin-6 (genetics)
• Keratinocytes (metabolism)
• Lactalbumin (administration & dosage, immunology)
• Lactoglobulins (administration & dosage, immunology)
• Macrophages (metabolism)
• Male
• Mast Cells (metabolism)
• Mice
• Mice, Inbred BALB C
• NF-kappa B
• RAW 264.7 Cells
• RNA, Messenger (analysis)
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Necrosis Factor-alpha (genetics)
• Thymic Stromal Lymphopoietin