Abstract | AIMS: This article aimed at discussing the association of chronic hepatitis B virus (HBV) infection with CD44 polymorphisms in Chinese Han population; meanwhile, the interaction of polymorphisms was also analyzed based on chronic HBV infection. METHODS: The genotyping of CD44 polymorphisms was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 108 HBV infected and 130 healthy persons. The genotype distributions of CD44 rs187115, rs13347 in the control group were checked by Hardy-Weinberg equilibrium (HWE). The strength of the relevance between polymorphism and disease was measured by odds ratio (OR) with corresponding 95% confidence interval (CI) calculated by χ(2) test. The 2×4 crossover analysis method was used to conduct the interaction analysis of polymorphisms. RESULTS: The genotype distributions in controls conformed to HWE. GG genotype and G allele frequencies in rs187115 were obviously higher in cases than the controls (P=0.02, 0.04). Compared with the common genotype CC, individual who carried mutant genotypes (CT and TT) of rs13347 had a significantly high risk to suffer from HBV infection (OR=1.99, P=0.02 for CT; OR=3.56, P=3.00×10(-3) for TT), furthermore, CT+TT genotype also showed a high susceptibility (OR=2.27, P=2.00×10(-3)). Similarly, T allele of rs13347 increased 0.98 times risk in cases compared with controls (OR=1.98, 95% CI=1.34-2.92). The two polymorphisms in CD44 presented a positive interaction. CONCLUSION: CD44 polymorphisms are associated with chronic HBV infection as the risk factors, and the synergistic action is also found between the two polymorphisms.
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Authors | Fan Li, Qing Shao, Dong Ji, Bing Li, Guofeng Chen |
Journal | International journal of clinical and experimental pathology
(Int J Clin Exp Pathol)
Vol. 8
Issue 9
Pg. 11675-9
( 2015)
ISSN: 1936-2625 [Electronic] United States |
PMID | 26617909
(Publication Type: Journal Article)
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Chemical References |
- CD44 protein, human
- Hyaluronan Receptors
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Topics |
- Adult
- Asian People
(genetics)
- Case-Control Studies
- Female
- Genetic Predisposition to Disease
(genetics)
- Genotype
- Hepatitis B, Chronic
(genetics)
- Humans
- Hyaluronan Receptors
(genetics)
- Male
- Middle Aged
- Odds Ratio
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single Nucleotide
- Young Adult
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