[Combined detection of thyroid transcription factor 1 and napsin A in pleural fluid cell blocks facilitates cytopathologic diagnosis of pulmonary adenocarcinoma].

Abstract

OBJECTIVE:

To investigate the value of detecting thyroid transcription factor 1 (TTF-1) and Noval aspartic proteinase of pepsin family A (napsin A) in pleural fluid cell blocks in cytopathologic diagnosis of pulmonary adenocarcinoma.

METHODS:

Conventional cell smears of pleural effusions were obtained from 48 patients with a history of lung adenocarcinoma for cytological analysis. The cell blocks were prepared using the cytological specimens and examined with immunohistochemistry for TTF-1 and napsin A. The rates of a positive diagnosis of pulmonary adenocarcinoma were compared between the two methods, and the diagnositic value of TTF-1 and napsin A in pleural fluid cell blocks was evaluated.

RESULTS:

Immuno- histochemistry of the cell block sections yielded a significantly higher positive rate of diagnosis than cytological analysis of conventional cell smear (84.44% vs 55.56%, P<0.05). Most of the pleural fluid cell blocks showed positive expressions of TTF-1 (36/38, 94.74%) and napsin A (30/38, 78.95%), and none of samples showed TTF-1 or napsin A expression in the mesothelial cells (P<0.05). The combination detection of TTF-1 and napsin A in pleural fluid cell blocks had a high diagnosis value with a diagnostic sensitivity of 97.37% and a specificity of 100% for pulmonary adenocarcinoma.

CONCLUSIONS:

The combined detection of TTF-1 and napsin A in pleural fluid cell blocks facilitates cytopathologic diagnosis of pulmonary adenocarcinoma.

• Authors: Xiaoyan Xu, Hongwei Liu, Huang Jiang, Chuan Li, Shuhui Yuan, Jinhua Yang
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 35 Issue 11 Pg. 1610-3 (Nov 2015) ISSN: 1673-4254 [Print] China
• PMID: 26607085 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• NKX2-1 protein, human
• Nuclear Proteins
• Thyroid Nuclear Factor 1
• Transcription Factors
• Aspartic Acid Endopeptidases
• NAPSA protein, human

Topics

• Adenocarcinoma (diagnosis, metabolism)
• Adenocarcinoma of Lung
• Aspartic Acid Endopeptidases (metabolism)
• Biomarkers, Tumor (metabolism)
• Humans
• Immunohistochemistry
• Lung Neoplasms (diagnosis, metabolism)
• Nuclear Proteins (metabolism)
• Pleural Effusion
• Sensitivity and Specificity
• Thyroid Nuclear Factor 1
• Transcription Factors (metabolism)