Abstract |
The induction by diethylsulphate of micronuclei derived from acentric fragments or from whole chromosomes was studied in Chinese hamster V79 cells using autoantibodies from the serum of a scleroderma patient ( CREST-syndrome) to detect centromere--kinetochore structures. Centromere-containing micronuclei appeared early after treatment and plateaued both earlier and at lower level than centromere-lacking micronuclei. The frequency of centromere-containing micronuclei was in good agreement with that of mitotic chromosome displacement, suggesting that a high proportion of displaced chromosomes were transmitted to the cytoplasm of one of the two daughter cells, where they gave rise to micronuclei. On the contrary, centromere-lacking micronuclei were more frequent than what could be expected from chromosome fragments observed in mitotic stages.
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Authors | M Nüsse, S Viaggi, S Bonatti |
Journal | Mutagenesis
(Mutagenesis)
Vol. 4
Issue 3
Pg. 174-8
(May 1989)
ISSN: 0267-8357 [Print] England |
PMID | 2659936
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Alkylating Agents
- Antibodies, Antinuclear
- Mutagens
- Sulfuric Acid Esters
- Sulfuric Acids
- diethyl sulfate
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Topics |
- Alkylating Agents
(toxicity)
- Animals
- Antibodies, Antinuclear
- Cell Division
- Cell Line
- Cell Nucleus
(drug effects, ultrastructure)
- Chromosome Aberrations
- Chromosomes
(drug effects, ultrastructure)
- DNA Repair
(drug effects)
- Micronucleus Tests
- Mutagens
- Sulfuric Acid Esters
(toxicity)
- Sulfuric Acids
(toxicity)
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