The present study attempts to investigate the anti-inflammatory potential of the isolated
lipid extracts of three-spot seahorse which is rare marine bony fish.
Petroleum ether (PE) extract was obtained from systematic
solvent extraction after reflux extraction with 95%
ethanol. FrIV was collected after
silica gel column chromatography, and neutral
lipids (NL),
glycolipids (GL),
phospholipids (PL) were separated from FrIV. Basic compositions were detected and analyzed via thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). Anti-inflammatory activities of total
lipids (TL), isolated NL, GL, and PL were detected by secretion of pro-inflammatory
cytokines induced by
lipopolysaccharide (LPS) in murine monocyte macrophage RAW264.7 cells in vitro. The results revealed that
lipids of seahorse showed a positive correlation with the in vitro suppression of the release of
nitric oxide (NO),
interleukin (IL)-6, IL-1β and
tumor necrosis factor (TNF)-α potently in a dose dependent manner, and showed cell compatibility. Among the fractions, GL (50 μg/mL) showed the highest capacity to attenuate the generation of pro-inflammatory
cytokines which was comparable to that of the positive
drug dexamethasone (DX) (20 μg/mL). Collectively, our findings indicated that the
lipids from seahorse may be effective in the management of
inflammation.