Knockdown of HNRNPA1 inhibits lung adenocarcinoma cell proliferation through cell cycle arrest at G0/G1 phase.

Abstract

Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), a member of heterogeneous nuclear ribonucleoprotein family in actively growing mammalian cells, is involved in a variety of RNA-related processes. HNRNPA1 can enhance the degradation of inhibitory subunit of nuclear factor κ B alpha (IκBα) and lengthen the telomeres. Recently, it is reported that HNRNPA1 is aberrantly expressed in varied tumors. In this study we found HNRNPA1 protein overexpressed in lung cancer tissues. To explore the exact role of HNRNPA1 in lung cancers, we carried out a loss of function analysis of HNRNPA1 in A549 lung cancer cells by RNA interference (RNAi). The results demonstrated that knockdown of HNRNPA1 inhibited cell viability and colony formation of lung cancer cells and arrested cell cycle in G0/G1 phase. Our study suggested that HNRNPA1 might play an important role in lung adenocarcinoma cells and provided a foundation for further study into the potential of HNRNPA1 for lung cancer therapy.

• Authors: Xianxun Liu, Yan Zhou, Yuqing Lou, Hua Zhong
• Journal: Gene (Gene) Vol. 576 Issue 2 Pt 2 Pg. 791-7 (Feb 01 2016) ISSN: 1879-0038 [Electronic] Netherlands
• PMID: 26581508 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Heterogeneous Nuclear Ribonucleoprotein A1
• Heterogeneous-Nuclear Ribonucleoprotein Group A-B
• RNA, Small Interfering
• hnRNPA1 protein, human

Topics

• Adenocarcinoma (genetics, pathology)
• Adenocarcinoma of Lung
• Aged
• Cell Line, Tumor
• Cell Proliferation
• Down-Regulation (genetics)
• Female
• Flow Cytometry
• G1 Phase Cell Cycle Checkpoints (genetics)
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• HEK293 Cells
• Heterogeneous Nuclear Ribonucleoprotein A1
• Heterogeneous-Nuclear Ribonucleoprotein Group A-B (genetics, metabolism)
• Humans
• Immunohistochemistry
• Lentivirus (metabolism)
• Lung Neoplasms (genetics, pathology)
• Male
• Middle Aged
• RNA, Small Interfering (metabolism)
• Tumor Stem Cell Assay