PDL regeneration via cell homing in delayed replantation of avulsed teeth.

Abstract

BACKGROUND:

This study was aimed to investigate whether regeneration of periodontal ligament (PDL) like tissue could be promoted by stromal cell-derived factor-1 (SDF1) and bone morphogenetic protein-7 (BMP7) induced cell homing in delayed replantation of avulsed teeth.

METHODS:

Canine mandibular premolar teeth were first extracted and air-dried for 2 h followed by complete detachment of their PDL tissues. The crown and pulp of the teeth were also removed. Twenty-four roots divided into two groups (n = 12/group) were used for the following in vivo transplantation. The roots of Group A were treated with 17 % EDTA for 24 h to achieve demineralization, and then coated with SDF1 and BMP7 supplemented collagen solution. The roots of Group B were similarly treated except being coated with a pristine collagen solution. The above roots were transplanted in the sockets that formed previously during tooth extraction. At 6 months' post-operation, PDL-like tissue composed of spindle-shaped cells, capillaries and highly organized collagen fibers was observed in the interstitial space between the avulsed root surface and surrounding alveolar bone in Group A. The neo-fibers inserted deeply and perpendicularly into the cementum and adjacent bone. The periodontium-like characteristics of the neo-tissue was confirmed by immunohistochemical staining for collagen I, fibronectin and osteocalcin.

RESULTS:

A high incidence of PDL re-establishment as 42 % was achieved for samples of Group A. However, no PDL-like tissue was found but root ankylosis and replacement resorption as well as inflammatory resorption was observed in the replanted roots of Group B.

CONCLUSIONS:

It can be confirmed that avulsed teeth could be successfully rescued even in delayed transplantation to avoid dentoalveolar ankylosis or replacement resorption via the current developed cell homing method.

• Authors: Wenting Zhu, Qian Zhang, Yang Zhang, Lian Cen, Jun Wang
• Journal: Journal of translational medicine (J Transl Med) Vol. 13 Pg. 357 (Nov 14 2015) ISSN: 1479-5876 [Electronic] England
• PMID: 26572489 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Dogs
• Immunohistochemistry
• Periodontal Ligament (metabolism, physiology)
• Regeneration
• Tooth Avulsion (surgery)
• Tooth Replantation