Tenascin-C (TN-C), an extracellular matrix (ECM)
glycoprotein, is specifically induced upon tissue injury and
infection and during septic conditions.
Carbon monoxide (CO) gas is known to exert various anti-inflammatory effects in various inflammatory diseases. However, the mechanisms underlying the effect of CO on TN-C-mediated
inflammation are unknown. In the present study, we found that treatment with LPS significantly enhanced TN-C expression in macrophages. CO gas, or treatment with the CO-donor compound,
CORM-2, dramatically reduced LPS-induced expression of TN-C and proinflammatory
cytokines while significantly increased the expression of
IL-10. Treatment with TN-C
siRNA significantly suppressed the effects of LPS on proinflammatory
cytokines production. TN-C
siRNA did not affect the CORM-2-dependent increase of
IL-10 expression. In cells transfected with
IL-10 siRNA,
CORM-2 had no effect on the LPS-induced expression of TN-C and its downstream
cytokines. These data suggest that
IL-10 mediates the inhibitory effect of CO on TN-C and the downstream production of proinflammatory
cytokines. Additionally, administration of
CORM-2 dramatically reduced LPS-induced TN-C and proinflammatory
cytokines production while expression of
IL-10 was significantly increased. In conclusion, CO regulated
IL-10 expression and thus inhibited TN-C-mediated
inflammation in vitro and in vivo.