Abstract | OBJECTIVE: METHOD: Using a small interference RNA ( siRNA) technology interfere β- catenin gene of Hep-2 cells . The mRNA and protein levels of β- catenin in the Hep-2 cells of different groups were detected by qPCR and Western blot. It was divided into siRNA-β-catenin-Hep-2 siRNA group, β- catenin-Neg negative control group and blank control group. Cell proliferation inhibition rate of different concentrations of cisplatin on three groups was detected by MTT assay. Calculate the 50% inhibitory effective concentration IC50 value. Check the change of three groups of cells' apoptosis rate by flow cytometry after the same concentrations of cisplatin stimulation. RESULT: β- catenin- siRNA interference fragment can specifically reduce the expression levels of β- catenin mRNA and protein. qPCR illustrated the expression of mRNA in β-catenin-siR-NA-Hep-2 interference group decreased 70% (P < 0.05) compared with the control group, Western blot results showed that the β- catenin protein expression of interference group (0. 545 ± 0.111) decreased significantly compared with blank control group (1.507 ± 0.139) and negative control group (1.429 ± 0.089), P < 0.05. The IC50 calculation software showed that IC50 of cisplatin on β- catenin- siRNA IC50 interference group is (5.81 ± 0.46)μg/ml, the blank control group is (10.10 ± 1.01) μg/ml, the difference between the two groups has statistical signifi- cance (P < 0.01). Cell apoptosis rate of β- catenin- siRNA interference group was (26.15 ± 0.60)%, significantly higher than the control group (14.16 ± 0.05)%, P < 0.05. CONCLUSION:
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Authors | Feng Yu, Xin Huang, Maomao Ai, Ying Lin |
Journal | Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery
(Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi)
Vol. 29
Issue 13
Pg. 1143-7
(Jul 2015)
ISSN: 2096-7993 [Print] China |
PMID | 26540910
(Publication Type: Journal Article)
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Chemical References |
- CTNNB1 protein, human
- RNA, Messenger
- RNA, Small Interfering
- beta Catenin
- Cisplatin
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Topics |
- Apoptosis
- Cell Line, Tumor
- Cisplatin
(pharmacology)
- Drug Resistance, Neoplasm
- Humans
- Laryngeal Neoplasms
(genetics)
- RNA Interference
- RNA, Messenger
- RNA, Small Interfering
- beta Catenin
(genetics)
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