Quinone-Modified Mn-Doped ZnS Quantum Dots for Room-Temperature Phosphorescence Sensing of Human Cancer Cells That Overexpress NQO1.

Abstract

Early detection of cancer cells in a rapid and sensitive approach is one of the great challenges in modern clinical cancer care. This study has demonstrated the first example of a rapid, selective, and sensitive phosphorescence probe based on phosphorescence energy transfer (PET) for cancer-associated human

NAD(P)H:

quinone oxidoreductase isozyme 1 (NQO1). An efficient room-temperature phosphorescence NQO1 probe was constructed by using Mn-doped ZnS quantum dots (Mn:ZnS QDs) as donors and trimethylquinone propionic acids as acceptors. Phosphorescence quenching of Mn:ZnS QDs from the Mn:ZnS QDs to a covalently bonded quinone was achieved through PET. Phosphorescence of Mn:ZnS QDs was turned on by the rapid reduction-initiated removal of the quinone quencher by NQO1. This probe shows low cellular toxicity and can rapidly distinguish between NQO1-expressing and -nonexpressing cancer cell lines through phosphorescence imaging.

• Authors: Yi-Ming Sung, Srivardhan Reddy Gayam, Pei-Ying Hsieh, Hsin-Yun Hsu, Eric Wei-Guang Diau, Shu-Pao Wu
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 7 Issue 46 Pg. 25961-9 (Nov 25 2015) ISSN: 1944-8252 [Electronic] United States
• PMID: 26540617 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Quinones
• Sulfides
• Zinc Compounds
• Manganese
• NAD(P)H Dehydrogenase (Quinone)
• NQO1 protein, human
• zinc sulfide

Topics

• Cell Line, Tumor
• Cell Survival
• Electrochemical Techniques
• Energy Transfer
• Humans
• Kinetics
• Luminescent Measurements (methods)
• Manganese (chemistry)
• NAD(P)H Dehydrogenase (Quinone) (metabolism)
• Quantum Dots (chemistry, ultrastructure)
• Quinones (chemistry)
• Spectroscopy, Fourier Transform Infrared
• Sulfides (chemistry)
• Temperature
• Time Factors
• Zinc Compounds (chemistry)