Timosaponin B-II inhibits lipopolysaccharide-induced acute lung toxicity via TLR/NF-κB pathway.

Abstract

Timosaponin B-II (TB), a main bioactive compound in Anemarrhena asphodeloides Bunge, has various kinds of pharmacological activities, the present study aimed to investigate the protective role of TB on lipopolysaccharide (LPS)-induced acute lung injury (ALI). ALI was induced in mice by intratracheal instillation of LPS, and TB (20 and 60 mg/kg) was given orally 1 h prior to LPS administration. After 6 h, bronchoalveolar lavagefluid (BALF) and lung tissue were collected. TB decreased LPS-induced evident lung histopathological changes, lung wet-to-dry weight (W/D) ratio and lung myeloperoxidase (MPO) activity. In addition, TB inhibited inflammatory cells and cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in BALF. Furthermore, we demonstrated that TB inhibited the Toll-like receptor-2 (TLR2), Toll-like receptor-4 (TLR4), myeloid differentiation primary response gene-88 (MyD88), nuclear factor-κB (NF-κB) p65 in LPS-induced ALI. These results showed that administration of TB prior to LPS improves ALI, possibly mediating ALI through suppressing TLR/NF-κB pathway activation.

• Authors: Tianzhu Zhang, Jing Wang, Shumin Wang, Chunhua Ma
• Journal: Toxicology mechanisms and methods (Toxicol Mech Methods) Vol. 25 Issue 9 Pg. 665-71 ( 2015) ISSN: 1537-6524 [Electronic] England
• PMID: 26540118 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Lipopolysaccharides
• Saponins
• Steroids
• Tlr2 protein, mouse
• Tlr4 protein, mouse
• Toll-Like Receptor 2
• Toll-Like Receptor 4
• Transcription Factor RelA
• lipopolysaccharide, E coli O55-B5
• timosaponin B-II

Topics

• Acute Lung Injury (chemically induced, metabolism, pathology, prevention & control)
• Animals
• Blotting, Western
• Bronchoalveolar Lavage Fluid (cytology, immunology)
• Cytokines (metabolism)
• Lipopolysaccharides (toxicity)
• Lung (drug effects, immunology, metabolism, pathology)
• Male
• Mice, Inbred BALB C
• Saponins (administration & dosage, therapeutic use)
• Signal Transduction (drug effects)
• Steroids (administration & dosage, therapeutic use)
• Toll-Like Receptor 2 (antagonists & inhibitors)
• Toll-Like Receptor 4 (antagonists & inhibitors)
• Transcription Factor RelA (antagonists & inhibitors)