Expression of interleukin-2 receptor beta subunit in hematopoietic malignancies.

Abstract

The expression of the interleukin-2 (IL-2) receptor was studied in neoplastic cells derived from acute leukemias, T-cell lymphoblastic lymphomas, peripheral T-cell lymphomas, chronic lymphocytic leukemias, well-differentiated lymphocytic lymphomas, and established cell lines by both flow cytometric analysis and sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) after affinity crosslinking of radiolabeled IL-2. Cells from most acute leukemias (19 of 22), irrespective of their subtype (T, common or nonlymphoid leukemias), as well as T-cell lymphoblastic lymphomas and peripheral T-cell lymphomas expressed only the p70-75 beta subunit of the IL-2 receptor. Cells from the more mature B-cell neoplasms, chronic lymphocytic leukemia, and well-differentiated lymphocytic lymphoma, expressed predominantly alpha beta IL-2 receptors (11 of 14). In contrast to these results, most cell lines established from hematopoietic malignancies do not express either chain of the IL-2 receptor. Further studies are necessary to determine the exact function of the IL-2R p70-75 beta subunit in immature hematopoietic cells, but its wide distribution throughout the hematopoietic system suggests that IL-2 may play a role in the early stages of hematopoiesis.

• Authors: A Rosolen, M Nakanishi, D G Poplack, D Cole, R Quinones, G Reaman, J B Trepel, J D Cotelingam, E A Sausville, G E Marti
• Journal: Blood (Blood) Vol. 73 Issue 7 Pg. 1968-72 (May 15 1989) ISSN: 0006-4971 [Print] United States
• PMID: 2653467 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cross-Linking Reagents
• Interleukin-2
• Receptors, Interleukin-2
• Recombinant Proteins

Topics

• Cell Line
• Cross-Linking Reagents
• Humans
• Interleukin-2 (metabolism)
• Leukemia (classification, metabolism)
• Leukemia, Lymphocytic, Chronic, B-Cell (analysis, metabolism)
• Leukemia-Lymphoma, Adult T-Cell (metabolism)
• Lymphoma (analysis, classification)
• Phenotype
• Receptors, Interleukin-2 (analysis, isolation & purification)
• Recombinant Proteins (metabolism)