[Effects of Different Dose of Cyclophosphamide on Peripheral Blood Cells of Normal ICR Mice].

Abstract

OBJECTIVE:

To investigate effective doses of cyclophosphamide (CTX) for establishment of leukopenia model in ICR mouse.

METHODS:

Normal ICR mice (n = 96) were divided into CTX(80), CTX(100), CTX(120), CTX(150) and CTX(300) groups, of which mice received CTX intraperitoneally at a dose of 80, 100, 120, or 150 mg/kg once a day for 3 consecutive days, or 300 mg/kg with single injection. The peripheral blood cell counts were detected at various times before and after CTX administration.

RESULTS:

The peripheral white blood cell nadirs in the mice injected with CTX appeared on day 4 after the first dose of CTX. The peripheral white blood cell nadir in group CTX(100) was 26.7% of the value measured in mice before CTX administrated, and that of group CTX(80) was 35.0%. Higher doses of CTX, however, caused too severity in hematopoietic injury.

CONCLUSION:

The dose of CTX 100 mg/(kg·d) × 3d is appropriate for leucopenia model of ICR mouse.

• Authors: Guo-Lin Xiong, Shuang Xing, Xing Shen, Meng Yang, Zu-Yin Yu
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 23 Issue 5 Pg. 1493-6 (Oct 2015) ISSN: 1009-2137 [Print] China
• PMID: 26524064 (Publication Type: Journal Article)

Chemical References

• Cyclophosphamide

Topics

• Animals
• Blood Cell Count
• Cyclophosphamide (administration & dosage)
• Leukocytes (drug effects)
• Mice
• Mice, Inbred ICR