Potential of four marine-derived fungi extracts as anti-proliferative and cell death-inducing agents in seven human cancer cell lines.

Abstract

OBJECTIVE:

To evaluate the in vitro anticancer activity of crude ethyl acetate extracts of the culture of four marine-derived fungi Aspergillus similanensis KUFA 0013 (E1), Neosartorya paulistensis KUFC 7897 (E2), Neosartorya siamensis KUFA 0017 (E4) and Talaromyces trachyspermus KUFC 0021 (E3) on a panel of seven human cancer cell lines.

METHODS:

Effects on cell proliferation, induction of DNA damage and cell death were assessed by MTT and clonogenic assays, comet assay and nuclear condensation assay, respectively.

RESULTS:

The proliferation of HepG2, HCT116 and A375 cells decreased after incubation with the extracts E2 and E4. The anti-proliferative effect was confirmed by morphologic alterations and by clonogenic assay. Both extracts also induced cell death in HepG2 and HCT116 cells. Doxorubicin was used as a positive control and showed in vitro anticancer activity.

CONCLUSIONS:

This study demonstrated, for the first time, that extracts of Neosartorya paulistensis and Neosartorya siamensis have selective anti-proliferative and cell death activities in HepG2, HCT16 and A375 cells. The bioactivity of these extracts suggests a potential for biotechnological applications and substantiates that both should be further considered for the elucidation of the molecular targets and signal transduction pathways involved.

• Authors: Alice Abreu Ramos, Maria Prata-Sena, Bruno Castro-Carvalho, Tida Dethoup, Suradet Buttachon, Anake Kijjoa, Eduardo Rocha
• Journal: Asian Pacific journal of tropical medicine (Asian Pac J Trop Med) Vol. 8 Issue 10 Pg. 798-806 (Oct 2015) ISSN: 2352-4146 [Electronic] India
• PMID: 26522294 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Hainan Medical College. Production and hosting by Elsevier B.V. All rights reserved.