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Differential expression of interleukin-17 and interleukin-22 in inflamed and non-inflamed synovium from osteoarthritis patients.

AbstractOBJECTIVE:
Synovitis associated with osteoarthritis (OA) is directly responsible for several clinical symptoms and reflects OA's structural progression. This study sought to analyze the expression of proinflammatory mediations, including Interleukin (IL)-17 and IL-22, which play key roles in regulating inflammatory processes, in inflamed and non-inflamed areas of osteoarthritic synovium.
METHODS:
Synovium from knees of 32 OA patients were collected at surgery. Macroscopic evaluation of inflammation enabled inflamed and non-inflamed areas to be separated. Samples were incubated to obtain tissue-conditioned media. Quantitative mRNA expression of proinflammatory mediators was analyzed by RT-PCR and protein levels by ELISA and gelatin zymography. Immunohistochemistry and histology were performed.
RESULTS:
Inflamed synovium were characterized by increased leukocyte infiltration and a higher vessel-to-tissue area ratio than non-inflamed tissues. Macrophages, T and B lymphocytes, and some neutrophils were found only in the inflamed tissue, and only in the subintimal layer. Levels of proinflammatory cytokines and MMP-9 were significantly higher in tissue-conditioned media from inflamed than non-inflamed tissues. Inflamed areas were associated with higher expression of IL-17 and IL-22, both correlated with the combined release of IL-6, IL-23, and TGFβ1.
CONCLUSION:
Our results showed that inflammatory cytokines, including IL-17 and IL-22, are expressed at higher levels by inflamed OA synovium and suggest IL-22 involvement in OA pathophysiology. This study will help identify new therapeutic strategies for OA, especially the targeting of IL-22 to decrease inflammation.
AuthorsC Deligne, S Casulli, A Pigenet, C Bougault, L Campillo-Gimenez, G Nourissat, F Berenbaum, C Elbim, X Houard
JournalOsteoarthritis and cartilage (Osteoarthritis Cartilage) Vol. 23 Issue 11 Pg. 1843-52 (Nov 2015) ISSN: 1522-9653 [Electronic] England
PMID26521730 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014. Published by Elsevier Ltd.
Chemical References
  • Interleukin-17
  • Interleukins
  • RNA
  • interleukin-22
Topics
  • Aged
  • Aged, 80 and over
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Gene Expression Regulation
  • Humans
  • Immunohistochemistry
  • Interleukin-17 (biosynthesis, genetics)
  • Interleukins (biosynthesis, genetics)
  • Male
  • Middle Aged
  • Osteoarthritis, Knee (complications, genetics, metabolism)
  • RNA (genetics)
  • Real-Time Polymerase Chain Reaction
  • Synovial Membrane (metabolism)
  • Synovitis (etiology, genetics, metabolism)

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