Effects of μ-Opioid Receptor Agonist DAMGO on Heart Contractility and Necrotic Injury to Cardiomyocytes during Ischemia and Reperfusion of Isolated Rat Heart.

Abstract

We studied the effects of μ-opioid receptor activation in vivo and in vitro on the tolerance of isolated perfused rat heart to global ischemia (45 min) and reperfusion (30 min). Stimulation of μ-receptors in vivo by intraperitoneal administration of μ-opioid receptor agonist DAMGO (0.1 mg/kg) reduced reperfusion release of creatinine phosphokinase and promoted aggravation of postischemic systolic and diastolic dysfunction of the isolated heart. Activation of μ-opioid receptors in vitro by addition of selective agonist DAMGO in a concentration of 170 nM to perfusion solution had no effect on necrotic death of cardiomyocytes and aggravated reperfusion stunning of the heart.

• Authors: T V Lasukova, L N Maslov, A S Gorbunov
• Journal: Bulletin of experimental biology and medicine (Bull Exp Biol Med) Vol. 159 Issue 6 Pg. 722-5 (Oct 2015) ISSN: 1573-8221 [Electronic] United States
• PMID: 26519265 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Opioid, mu
• Enkephalin, Ala(2)-MePhe(4)-Gly(5)-

Topics

• Animals
• Cell Death (drug effects)
• Enkephalin, Ala(2)-MePhe(4)-Gly(5)- (pharmacology, therapeutic use)
• Heart (drug effects, physiology)
• Male
• Myocardial Contraction (drug effects)
• Myocardial Ischemia (pathology, physiopathology)
• Myocardial Reperfusion Injury (pathology, physiopathology)
• Myocardium (pathology)
• Myocytes, Cardiac (drug effects, pathology)
• Necrosis
• Organ Culture Techniques
• Rats
• Rats, Wistar
• Receptors, Opioid, mu (agonists)
• Reperfusion