Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan--Quantitative Shiga toxin detection from stool during EHEC outbreak.

Abstract

Detection of Shiga toxins (Stx) is important for accurate diagnosis of Enterohemorrhagic Escherichia coli infection. In this study, we quantitatively analyzed Stx protein in nine patients' stool during an outbreak that occurred in Japan. Highly sensitive immunoassay (bead enzyme-linked immunosorbent assay (bead-ELISA)) revealed that the concentrations of toxins in stool of patients ranged from 0.71 to 10.44 ng/mL for Stx1 and 2.75 to 51.61 ng/mL for Stx2. To our knowledge, this is the first report that reveals the range of Stx protein concentrations in human stools.

• Authors: Eiki Yamasaki, Masanori Watahiki, Junko Isobe, Tetsutaro Sata, G Balakrish Nair, Hisao Kurazono
• Journal: Toxins (Toxins (Basel)) Vol. 7 Issue 10 Pg. 4381-9 (Oct 27 2015) ISSN: 2072-6651 [Electronic] Switzerland
• PMID: 26516915 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Shiga Toxins

Topics

• Adolescent
• Adult
• Child
• Child, Preschool
• Disease Outbreaks
• Enterohemorrhagic Escherichia coli (isolation & purification)
• Enzyme-Linked Immunosorbent Assay
• Escherichia coli Infections (epidemiology, microbiology)
• Feces (chemistry)
• Female
• Humans
• Infant
• Japan (epidemiology)
• Male
• Middle Aged
• Shiga Toxins (analysis, genetics)
• Young Adult