Abstract | BACKGROUND/AIMS: METHODS: RESULTS: Lv-GFP-sPLA2-IIA-transduced HASMCs remained fluorescent during 72 h of the study period with infection ratio of around 80%. The mRNA expression and protein secretion of MCP-1 was not altered in groups of HASMCs, Lv-GFP transduced and Lv-GFP-sPLA2-IIA-transduced HASMCs (p>0.05), but was significantly increased in the presence of oxidized LDL especially in Lv-GFP-sPLA2-IIA transduction group (p<0.01). However, with the addition of LY315920, this enhancement was notably decreased (p<0.05). This enhancement was also markedly abolished by co-incubation with LY294002, paralleled with suppressed Akt phosphorylation. CONCLUSIONS: Overexpression of sPLA2-IIA does not alter MCP-1 level at baseline, but could enhance the atherogenic effect of oxidized LDL in HASMCs, at least partly due to activation of Akt. These findings may provide a strategy for treatment of inflammatory cardiovascular diseases.
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Authors | Yongjun Guo, Bo Li, Xuejing Xu, Rong Wu, Weihua Li |
Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
(Cell Physiol Biochem)
Vol. 37
Issue 4
Pg. 1345-54
( 2015)
ISSN: 1421-9778 [Electronic] Germany |
PMID | 26488172
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 S. Karger AG, Basel. |
Chemical References |
- Acetates
- CCL2 protein, human
- Chemokine CCL2
- Chromones
- Indoles
- Keto Acids
- Lipoproteins, LDL
- Morpholines
- Phosphoinositide-3 Kinase Inhibitors
- RNA, Messenger
- oxidized low density lipoprotein
- varespladib methyl
- 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
- Proto-Oncogene Proteins c-akt
- Group II Phospholipases A2
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Topics |
- Acetates
(pharmacology)
- Cell Line
- Chemokine CCL2
(metabolism)
- Chromones
(pharmacology)
- Enzyme-Linked Immunosorbent Assay
- Gene Expression
(drug effects)
- Genes, Reporter
- Group II Phospholipases A2
(antagonists & inhibitors, genetics, metabolism)
- HEK293 Cells
- Humans
- Indoles
(pharmacology)
- Keto Acids
- Lipoproteins, LDL
(toxicity)
- Morpholines
(pharmacology)
- Myocytes, Smooth Muscle
(cytology, metabolism)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Phosphoinositide-3 Kinase Inhibitors
- Phosphorylation
- Proto-Oncogene Proteins c-akt
(antagonists & inhibitors, metabolism)
- RNA, Messenger
(metabolism)
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