Abstract |
Metagenome research has brought much attention for the identification of important and novel genes of industrial and pharmaceutical value. Here, using a metagenome library constructed from bacteria associated with the marine sponge, Styllisa massa, a high-throughput screening technique using radioisotope was implemented to screen for cadmium (Cd) binding or accumulation genes. From a total of 3301 randomly selected clones, a clone 247-11C was identified as harboring an open reading frame (ORF) showing Cd accumulation characteristics. The ORF, termed as ORF5, was further analyzed by protein functional studies to reveal the presence of a protein, Cdae-1. Cdae-1, composed of a signal peptide and domain harboring an E(G/A)KCG pentapeptide motif, enhanced Cd accumulation when expressed in Escherichia coli. Although showing no direct binding to Cd in vitro, the presence of important amino acid residues related to Cd detoxification suggests that Cdae-1 may possess a different mechanism from known Cd binding proteins such as metallothioneins (MTs) and phytochelatins (PCs). In summary, using the advantage of bacterial metagenomes, our findings in this work suggest the first report on the identification of a unique protein involved in Cd accumulation from bacteria associated with a marine sponge.
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Authors | Tetsushi Mori, Koji Iwamoto, Satoshi Wakaoji, Hiroya Araie, Yotaro Kohara, Yoshiko Okamura, Yoshihiro Shiraiwa, Haruko Takeyama |
Journal | Gene
(Gene)
Vol. 576
Issue 2 Pt 1
Pg. 618-25
(Feb 01 2016)
ISSN: 1879-0038 [Electronic] Netherlands |
PMID | 26484790
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier B.V. All rights reserved. |
Chemical References |
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Topics |
- Amino Acid Sequence
- Base Sequence
- Cadmium
(metabolism)
- Genome, Bacterial
- Metagenome
- Molecular Sequence Data
- Sequence Homology, Amino Acid
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