Abstract |
Polyreactive antibodies are a major component of the natural antibody repertoire and bind to a variety of structurally unrelated molecules. These antibodies are thought to provide a first line of defense against bacterial infections and play a major role in the clearance of apoptotic cells. What triggers the secretion of these antibodies has remained an enigma. Using a surrogate assay for measuring polyreactive antibodies, we found that about 50% of serum IgM is polyreactive and that stimulation of TLR4(+/+), but not TLR4(-/-), mice resulted in a 40 fold increase in polyreactive antibodies. Stimulation of TLRs 3, 7, 9 also increased the secretion of polyreactive antibodies. Infection with a virus or tissue damage induced by a toxin similarly led to an increase in polyreactive antibodies in MyD88(+/+), but not MyD88(-/-) mice. We conclude that stimulation of TLRs is a key link in the mechanism of polyreactive antibody secretion into the circulation.
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Authors | Sreenivasulu Gunti, Ronald J Messer, Chengfu Xu, Ming Yan, William G Coleman Jr, Karin E Peterson, Kim J Hasenkrug, Abner L Notkins |
Journal | Scientific reports
(Sci Rep)
Vol. 5
Pg. 15066
(Oct 14 2015)
ISSN: 2045-2322 [Electronic] England |
PMID | 26463758
(Publication Type: Journal Article, Research Support, N.I.H., Intramural)
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Chemical References |
- Autoantibodies
- Toll-Like Receptors
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Topics |
- Animals
- Autoantibodies
(blood, immunology)
- Female
- Immunity, Innate
(immunology)
- Immunization
(methods)
- Mice
- Mice, Inbred C57BL
- Toll-Like Receptors
(immunology)
- Up-Regulation
(immunology)
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