Abstract | INTRODUCTION: Enterococcus faecalis is a frequently isolated microorganism in persistent periapical lesion or secondary infection. However, no evidence has demonstrated that E. faecalis induced inflammation directly in the apical area. This study aimed to explore the mechanism of the inflammatory responses of human periodontal ligament fibroblasts (PDLs) to E. faecalis. METHODS: RESULTS: Both HKEF and LTA stimulated the expression of toll-like receptor 2 and TRAF6 in a time-dependent manner. The secretions of proinflammatory cytokines were also increased. After silencing TRAF6, the upregulations of proinflammatory cytokines induced by HKEF or LTA were attenuated. CONCLUSIONS:
TRAF6 plays a pivotal role in inflammation induced by E. faecalis or its LTA in PDLs.
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Authors | Lan Zhang, Tingting Wang, Yu Lu, Qinghua Zheng, Yuan Gao, Xuedong Zhou, Dingming Huang |
Journal | Journal of endodontics
(J Endod)
Vol. 41
Issue 12
Pg. 1997-2001
(Dec 2015)
ISSN: 1878-3554 [Electronic] United States |
PMID | 26454720
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Lipopolysaccharides
- TNF Receptor-Associated Factor 6
- Teichoic Acids
- lipoteichoic acid
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Topics |
- Cells, Cultured
- Enterococcus faecalis
(physiology)
- Fibroblasts
(immunology)
- Humans
- Immunity, Innate
- Lipopolysaccharides
(immunology)
- Periapical Periodontitis
(immunology, microbiology)
- Periodontal Ligament
(immunology, microbiology)
- TNF Receptor-Associated Factor 6
(immunology)
- Teichoic Acids
(immunology)
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