Abstract |
Sera collected from patients with suspected or confirmed exposure to Trichinella spiralis were tested for circulating parasite antigens and antiparasite antibodies. Using an immunoradiometric assay, excretory--secretory antigens from muscle-stage larvae of T. spiralis were detected in the sera of 47% of 62 patients with clinical trichinellosis and 13% of 39 patients without clinical signs but suspected of exposure to infected meat. In comparison, antibodies were detected using an indirect immunofluorescent test in the circulation of 100% of the 62 patients with clinical trichinellosis and 46% of the 39 patients with suspected exposure. The presence of antibodies specific to excretory-secretory products of T. spiralis muscle larvae was confirmed in the majority of the samples tested by a monoclonal antibody-based competitive inhibition assay. These results indicate that antibody detection is a more sensitive diagnostic method for human trichinellosis, but that antigen detection might be a useful confirmatory test because it is a direct demonstration of parasite products in the circulation.
|
Authors | D Ivanoska, K Cuperlović, H R Gamble, K D Murrell |
Journal | The Journal of parasitology
(J Parasitol)
Vol. 75
Issue 1
Pg. 38-41
(Feb 1989)
ISSN: 0022-3395 [Print] United States |
PMID | 2645393
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
|
Chemical References |
- Antibodies, Helminth
- Antigens, Helminth
- Iodine Radioisotopes
|
Topics |
- Antibodies, Helminth
(analysis)
- Antigens, Helminth
(analysis)
- Fluorescent Antibody Technique
- Humans
- Iodine Radioisotopes
- Serologic Tests
(methods)
- Trichinellosis
(diagnosis)
|