Abstract |
Infection with Plasmodium falciparum parasites causes the majority of malaria-related morbidity and mortality. Constant exposure to the pathogen leads to the acquisition of antibodies and high levels of antibodies have been associated with clinical protection against malaria. A possible protective mechanism is the opsonization of parasites, or malaria-infected erythrocytes (IEs), for phagocytic clearance. Current assays use adherent or chemically differentiated THP-1 cells to evaluate opsonic antibodies in patients' samples, but these assays are often time consuming and damage the effector cells. We have developed a high throughput flow cytometry-based phagocytosis assay using undifferentiated THP-1 cells to quantify the opsonic activity against late stage P. falciparum-IEs. Opsonic antibodies bound to IEs promote their phagocytic uptake through Fcγ receptors found on THP-1 cells. Moreover, undifferentiated THP-1 cells do not express CD36, a surface scavenger receptor that promotes non-opsonic phagocytosis. This technical advance allows quantification of opsonic antibodies and is an important tool for the performance of large, population-based studies of malaria immunity, and to provide a significant increase in the statistical power for such studies.
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Authors | Andrew Teo, Wina Hasang, Philippe Boeuf, Stephen Rogerson |
Journal | Methods in molecular biology (Clifton, N.J.)
(Methods Mol Biol)
Vol. 1325
Pg. 145-52
( 2015)
ISSN: 1940-6029 [Electronic] United States |
PMID | 26450386
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Protozoan
- Antigens, Protozoan
- Immunoglobulin G
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Topics |
- Antibodies, Protozoan
(blood, immunology)
- Antigens, Protozoan
(blood, immunology)
- Erythrocytes
(immunology, parasitology)
- Flow Cytometry
(methods)
- Humans
- Immunoglobulin G
(blood)
- Malaria, Falciparum
(immunology, parasitology)
- Monocytes
(immunology)
- Phagocytosis
(immunology)
- Plasmodium falciparum
(immunology, pathogenicity)
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