Evaluation of (99m)Tc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice.

Abstract

Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using (99m)Tc-HYNIC-βAla-Bombesin(7-14)as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods showed higher uptake by pancreatic tumor when compared to muscle (used as control), and the tumor-to-muscle ratio indicated that (99m)Tc-HYNIC-βAla-Bombesin (7-14)uptake was four-fold higher in tumor cells than in other tissues. Scintigraphic images also showed a clear signal at the tumor site. The present data indicate that (99m)Tc-HYNIC-βAla-Bombesin (7-14) may be useful for the detection of pancreatic adenocarcinoma.

• Authors: F N Carlesso, L L Fuscaldi, R S Araújo, C S Teixeira, M C Oliveira, S O A Fernandes, G D Cassali, D C Reis, A L B Barros, V N Cardoso
• Journal: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (Braz J Med Biol Res) Vol. 48 Issue 10 Pg. 923-8 (Oct 2015) ISSN: 1414-431X [Electronic] Brazil
• PMID: 26445336 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Organotechnetium Compounds
• Peptide Fragments
• technetium 99m HYNIC-beta-Ala-bombesin(7-14)
• Gastrin-Releasing Peptide
• Bombesin

Topics

• Adenocarcinoma (diagnostic imaging, pathology)
• Animals
• Bombesin (analogs & derivatives, pharmacokinetics)
• Cell Line, Tumor
• Gastrin-Releasing Peptide (analogs & derivatives)
• Heterografts (diagnostic imaging, pathology)
• Humans
• Male
• Mice, Nude
• Muscles (diagnostic imaging)
• Organotechnetium Compounds (pharmacokinetics)
• Pancreatic Neoplasms (diagnostic imaging, pathology)
• Peptide Fragments (pharmacokinetics)
• Radionuclide Imaging