Circulating microparticle signature in coronary and peripheral blood of ST elevation myocardial infarction patients in relation to pain-to-PCI elapsed time.

Abstract

BACKGROUND:

Circulating microparticle (cMP) levels are increased in the acute phase of ST-elevation myocardial infarction (STEMI) and associate with microvascular obstruction; however, the precise cMP-parental cell signature and activation level are not elucidated. Here, we aimed to study the cMP signature in STEMI-patients and whether cMP phenotype changes in relation to onset of pain-to-PCI [ischemic time (IT)]-elapsed time.

METHODS:

Blood was taken at PCI from the culprit coronary and the peripheral circulation in STEMI-patients (N=40). Two control groups were included: peripheral blood of age-matched patients recovering from STEMI [after 72 h] and of control individuals (N=20/group). cMP-parental origin and activation level were characterized by triple-labeling flow cytometry.

RESULTS:

Procoagulant annexin V-positive cMPs bearing parental cell markers as well as markers of activated cells displayed a significantly different profile in STEMI-patients, in control individuals and in patients recovering from STEMI. cMPs derived from monocytes, endothelium, and activated vascular cells were higher in the culprit coronary artery than in peripheral blood in STEMI-patients, especially in patients intervened at short IT. Indeed, cMP levels in coronary blood were inversely related to IT duration (more abundant in thrombi with pain-to-PCI time<180 min).

CONCLUSIONS:

A characteristic [CD66b+/CD62E+/CD142+] cMP signature in the systemic circulation reflects the formation of coronary thrombotic occlusions in STEMI-patients. Changes in the cMP signature in the culprit coronary artery blood reveal the sensitivity of MPs to detect the ischemia-elapsed time. Interestingly, cMPs in peripheral blood may be sensitive markers of the thrombo-occlusive vascular process developing in the coronary arteries of STEMI-patients.

• Authors: R Suades, T Padró, J Crespo, I Ramaiola, V Martin-Yuste, M Sabaté, J Sans-Roselló, A Sionis, L Badimon
• Journal: International journal of cardiology (Int J Cardiol) Vol. 202 Pg. 378-87 (Jan 01 2016) ISSN: 1874-1754 [Electronic] Netherlands
• PMID: 26432487 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Biomarkers

Topics

• Aged
• Angioplasty, Balloon, Coronary (methods)
• Biomarkers (blood, metabolism)
• Cell-Derived Microparticles (metabolism)
• Coronary Occlusion (blood, metabolism)
• Coronary Vessels (metabolism)
• Electrocardiography
• Female
• Flow Cytometry (methods)
• Humans
• Male
• Middle Aged
• Myocardial Infarction (blood, metabolism, surgery)
• Myocardial Revascularization (methods)
• Pain (diagnosis, metabolism, surgery)
• Percutaneous Coronary Intervention (methods)
• Phenotype
• Predictive Value of Tests
• Severity of Illness Index
• Thrombectomy (methods)
• Thrombosis (metabolism)