Electron microscopic aspects of the effects of certain prostaglandin analogs on mouse testes.

Abstract

Prostaglandins were highlighted in the seminal plasma and then in the rest of the male and female genital tract. Prostaglandin analogs, firstly used in obstetrics and gynecology, are now widespread in both sexes, especially in the treatment of gastric and duodenal ulcers, glaucoma, etc. Therefore, we tried to highlight the effects of repeated administration of Cloprostenol and CIPG isopropyl ester (both prostaglandin F2α analogs) for the male gonad. In our experiment, we used Cloprostenol and CIPG isopropyl ester. We used three groups of white, male mice, aged 50-80 days, kept in standard laboratory conditions, which received the same feed. Each group included 12 mice. The first batch was the control group and received no substance at all. The second batch received 25 μg/kg of Cloprostenol dose per body per day, intraperitoneal administration (a single dose per day) on a daily basis for a four weeks period of time. The third batch received a 25 μg/kg CIPG isopropyl ester dose per body/day intraperitoneal administration (a single dose per day) on a daily basis for a four weeks period of time. After 7, 14 and 28 days of treatment, we sacrificed four animals in each of the batches by cutting their carotid arteries. The prostanoid analogs we used, Cloprostenol and CIPG isopropyl ester, have similar actions on male gonad in mice. These analogs induced significant changes in the evolution of the spermatogenesis and spermiogenesis. In relation to the treatment duration there were cellular changes suggesting apoptosis in different stages. With regard to spermiogenesis, the ultrastructural aspects indicate a decrease of the sperm structuring processes, especially in the acrosomal apparatus and chromatin.

• Authors: Anca Sava, Andrei Gheorghe Marius Motoc, Cristinel Ionel Stan
• Journal: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (Rom J Morphol Embryol) Vol. 56 Issue 2 Suppl Pg. 771-5 ( 2015) ISSN: 2066-8279 [Electronic] Romania
• PMID: 26429171 (Publication Type: Journal Article)

Chemical References

• 3-(5-cyano-6-(isopropylamino)pyrimidin-4-ylamino)-N-(isoxazol-3-yl)-4-methylbenzamide
• Benzamides
• Chromatin
• Prostaglandins
• Prostaglandins, Synthetic
• Pyrimidines
• Cloprostenol

Topics

• Animals
• Apoptosis
• Benzamides
• Carotid Arteries (pathology, ultrastructure)
• Chromatin (metabolism)
• Cloprostenol (analogs & derivatives, therapeutic use)
• Infusions, Parenteral
• Male
• Mice
• Microscopy, Electron
• Prostaglandins (chemistry)
• Prostaglandins, Synthetic (therapeutic use)
• Pyrimidines
• Spermatogenesis (drug effects)
• Testis (drug effects, ultrastructure)