Cell Fate of Müller Cells During Photoreceptor Regeneration in an N-Methyl-N-nitrosourea-Induced Retinal Degeneration Model of Zebrafish.

Abstract

Zebrafish can regenerate several organs such as the tail fin, heart, central nervous system, and photoreceptors. Very recently, a study has demonstrated the photoreceptor regeneration in the alkylating agent N-methyl-N-nitrosourea (MNU)-induced retinal degeneration (RD) zebrafish model, in which whole photoreceptors are lost within a week after MNU treatment and then regenerated within a month. The research has also shown massive proliferation of Müller cells within a week. To address the question of whether proliferating Müller cells are the source of regenerating photoreceptors, which remains unknown in the MNU-induced zebrafish RD model, we employed a BrdU pulse-chase technique to label the proliferating cells within a week after MNU treatment. As a result of the BrdU pulse-chase technique, a number of BrdU(+) cells were observed in the outer nuclear layer as well as the inner nuclear layer. This implies that regenerating photoreceptors are derived from proliferating Müller cells in the zebrafish MNU-induced RD model.

• Authors: Kazuhiro Ogai, Suguru Hisano, Kayo Sugitani, Yoshiki Koriyama, Satoru Kato
• Journal: Advances in experimental medicine and biology (Adv Exp Med Biol) Vol. 854 Pg. 685-92 ( 2016) ISSN: 0065-2598 [Print] United States
• PMID: 26427476 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proliferating Cell Nuclear Antigen
• Methylnitrosourea
• Bromodeoxyuridine

Topics

• Animals
• Bromodeoxyuridine (metabolism)
• Cell Proliferation
• Disease Models, Animal
• Ependymoglial Cells (metabolism, physiology)
• Female
• Immunohistochemistry
• Male
• Methylnitrosourea
• Photoreceptor Cells, Vertebrate (metabolism, physiology)
• Proliferating Cell Nuclear Antigen (metabolism)
• Regeneration (physiology)
• Retinal Degeneration (chemically induced, metabolism, physiopathology)
• Zebrafish