Abstract |
In common with most mammals, humans form only two dentitions during their lifetime. Occasionally, supernumerary teeth develop in addition to the normal complement. Odontoma represent a small group of malformations containing calcified dental tissues of both epithelial and mesenchymal origin, with varying levels of organization, including tooth-like structures. The specific cell type responsible for the induction of odontoma, which retains the capacity to re-initiate de novo tooth development in postnatal tissues, is not known. Here we demonstrate that aberrant activation of WNT signaling by expression of a non-degradable form of β- catenin specifically in SOX2-positive postnatal dental epithelial stem cells is sufficient to generate odontoma containing multiple tooth-like structures complete with all dental tissue layers. Genetic lineage-tracing confirms that odontoma form in a similar manner to normal teeth, derived from both the mutation-sustaining epithelial stem cells and adjacent mesenchymal tissues. Activation of the WNT pathway in embryonic SOX2-positive progenitors results in ectopic expression of secreted signals that promote odontogenesis throughout the oral cavity. Significantly, the inductive potential of epithelial dental stem cells is retained in postnatal tissues, and up-regulation of WNT signaling specifically in these cells is sufficient to promote generation and growth of ectopic malformations faithfully resembling human odontoma.
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Authors | Guilherme M Xavier, Amanda L Patist, Chris Healy, Ankita Pagrut, Gabriela Carreno, Paul T Sharpe, Juan Pedro Martinez-Barbera, Selvam Thavaraj, Martyn T Cobourne, Cynthia L Andoniadou |
Journal | Scientific reports
(Sci Rep)
Vol. 5
Pg. 14479
(Sep 28 2015)
ISSN: 2045-2322 [Electronic] England |
PMID | 26411543
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CTNNB1 protein, mouse
- SOXB1 Transcription Factors
- Wnt Proteins
- beta Catenin
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Topics |
- Animals
- Cell Differentiation
- Cell Transformation, Neoplastic
(genetics, metabolism)
- Embryonic Stem Cells
(metabolism)
- Female
- Gene Expression
- Male
- Mice
- Odontogenesis
(genetics)
- Odontoma
(genetics, metabolism, pathology)
- Pregnancy
- SOXB1 Transcription Factors
(genetics, metabolism)
- Stem Cells
(metabolism)
- Wnt Proteins
(genetics, metabolism)
- Wnt Signaling Pathway
- beta Catenin
(metabolism)
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