Abstract | BACKGROUND: METHODS: Twenty-four patients, 12 in the first and 12 in the second stage of NAFLD, were prospectively enrolled in this study. Biochemical parameters and eicosanoids ( HETE and HODE) were compared between the first and the second stage of hepatic steatosis and the effect of a 6-month dietary intervention on these parameters was evaluated. Eicosanoid profiles were extracted from 0.5 ml of plasma using solid-phase extraction RP-18 SPE columns. The HPLC separations were performed on a 1260 liquid chromatograph. RESULTS: Patients with stage I NAFLD had a significantly higher level of HDL cholesterol and a lower level of 5-HETE. Patients with grade II steatosis had higher concentrations of 9-HODE. Following the six-month dietary intervention, hepatic steatosis resolved completely in all patients. This resulted in a significant decrease in the concentrations of all eicosanoids (LX4, 16-HETE, 13-HODE, 9-HODE, 15-HETE, 12-HETE, 5-oxoETE, 5-HETE) and key biochemical parameters (BMI, insulin, HOMA-IR, liver enzymes). CONCLUSION: A significant reduction in the analyzed eicosanoids and a parallel reduction in fatty liver confirmed the usefulness of HETE and HODE in the assessment of NAFLD.
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Authors | Dominika Maciejewska, Piotr Ossowski, Arleta Drozd, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Joanna Raszeja-Wyszomirska, Ewa Stachowska |
Journal | Prostaglandins & other lipid mediators
(Prostaglandins Other Lipid Mediat)
Vol. 121
Issue Pt B
Pg. 184-9
(Sep 2015)
ISSN: 1098-8823 [Print] United States |
PMID | 26408952
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015. Published by Elsevier Inc. |
Chemical References |
- Arachidonic Acids
- Biomarkers
- Linoleic Acids
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Topics |
- Adult
- Arachidonic Acids
(blood, metabolism)
- Biomarkers
(blood)
- Body Mass Index
- Diet, Mediterranean
- Diet, Reducing
- Down-Regulation
- Early Diagnosis
- Female
- Humans
- Linoleic Acids
(blood, metabolism)
- Liver
(diagnostic imaging, metabolism)
- Male
- Middle Aged
- Non-alcoholic Fatty Liver Disease
(diagnostic imaging, diet therapy, metabolism, physiopathology)
- Overweight
(complications, diet therapy)
- Oxidative Stress
- Pilot Projects
- Prospective Studies
- Remission Induction
- Severity of Illness Index
- Ultrasonography
- Up-Regulation
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