Photodynamic inactivation of virulence factors of Candida strains isolated from patients with denture stomatitis.

Abstract

Candida species are major microorganisms isolated in denture stomatitis (DS), an inflammatory process of the mucosa underlying removable dental prostheses, and express a variety of virulence factors that can increase their pathogenicity. The potential of Photodynamic inactivation (PDI) in planktonic culture, biofilms and virulence factors of Candida strains was evaluated. A total of 48 clinical Candida isolates from individuals wearing removable maxillary prostheses with DS were included in the study. The effects of erythrosine (ER, 200 μM) and a green LED (λ 532 ± 10 nm, 237 mW/cm(2) and 42.63 J/cm(2)) in a planktonic culture were evaluated. The effect of the addition of ER at a concentration of 400 μM together with a green LED was evaluated in biofilms. The virulence factors of all of the Candida strains were evaluated before and after the PDI process in cells derived from biofilm and planktonic assays. All of the Candida species were susceptible to ER and green LED. However, the biofilm structures were more resistant to PDI than the planktonic cultures. PDI also promoted slight reductions in most of the virulence factors of C. albicans and some of the Candida tropicalis strains. These results suggest that the addition of PDI is effective for reducing yeasts and may also reduce the virulence of certain Candida species and decrease their pathogenicity.

• Authors: Cristiane Aparecida Pereira, Nádia Domingues, Michelle Peneluppi Silva, Anna Carolina Borges Pereira Costa, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge
• Journal: Journal of photochemistry and photobiology. B, Biology (J Photochem Photobiol B) Vol. 153 Pg. 82-9 (Dec 2015) ISSN: 1873-2682 [Electronic] Switzerland
• PMID: 26398815 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Photosensitizing Agents
• Virulence Factors
• Erythrosine

Topics

• Biofilms (drug effects, radiation effects)
• Candida (isolation & purification, physiology)
• Erythrosine (chemistry, pharmacology)
• Humans
• Light
• Photosensitizing Agents (chemistry, pharmacology)
• Stomatitis, Denture (microbiology, pathology)
• Virulence Factors (metabolism)