Due to the irregular of diet and overfeeding greasy and surfeit flavor closely associated with
hyperuricemia disease, the
lipid emulsion containing high
cholesterol was used to model. To obtain a more stable and sustained animal model for the efficacy evaluation of traditional Chinese herbs, we observed the influence on the serum
uric acid of rat induced by the
lipid emulsion compared with high
purine diet. 36 SD male rats were randomized to the normal control group, high
purine diet group and
lipid emulsion group respectively. The general behavior,
body weight and daily food intake of rats were observed. The orbital blood was taken to separate into the serum and 24 hours urine was collected. The serum indexes such as UA, BUN, Cr, ALT, AST, TC, TG,
LDL-c were determined every 2 weeks, and XOD, ADA
enzyme activity were determined at the 4th week. The urine indexes such as UA, Cr and Cua/Ccr were determined at the 4th week. After stopping modeling, the serum UA were determined two weeks and four weeks later respectively. At the 2nd week, the
body weight and daily food intake of rats in the
lipid emulsion group reduced significantly, and the level of serum UA, BUN, Cr, TC,
LDL-c, ATL, AST raised significantly meanwhile TG reduced. At the 4th week, the serum UA in high
purine diet group did not raise, and the serum XOD raised obviously while ADA did not; the serum UA in
lipid emulsion group was higher significantly, and the serum XOD and ADA raised while Cua/Ccr reduced obviously. At the 6th weeks, the serum UA in both the high
purine diet group and
lipid emulsion group raised obviously. After stopping modeling, the serum UA in
lipid emulsion group still maintained a high level at the 2nd week and back to the normal level at the 4th week. Compared with high
purine diet, the
hyperuricemia model induced by
lipid emulsion forms earlierand more stable. It maybe has great value to study the pharmacodynamics of
traditional Chinese medicine treatment to
hyperuricemia disease. Its mechanism may be related to increasing XOD and ADA
enzyme activity which can promote
uric acid synthesis, meanwhile inhibiting of
uric acid excretion.