Treatment of perianal fistulas with human embryonic stem cell-derived mesenchymal stem cells: a canine model of human fistulizing Crohn's disease.

Abstract

AIM:

To evaluate the safety and efficacy of intralesional injection of human embryonic stem cell (hESC)-derived mesenchymal stem/stromal cells (MSCs) in canine anal furunculosis dogs.

MATERIALS & METHODS:

Dogs naturally develop an immune-mediated disease called canine anal furunculosis, which shares many features with human fistulizing Crohn's disease.

RESULTS:

The hESC-MSCs were well tolerated and 1 month postinjection, accompanied by reduced serum levels of IL-2 and IL-6, two inflammatory cytokines associated with Crohn's disease. All six dogs were found to be completely free of fistulas at 3 months postinjection. However, at 6 months, two dogs had some fistula relapse.

CONCLUSION:

Results of this study provide the first evidence of the safety and therapeutic potential of hESC-MSCs in a large animal model.

• Authors: Lluís Ferrer, Erin A Kimbrel, Andrea Lam, Elizabeth B Falk, Christine Zewe, Tarja Juopperi, Robert Lanza, Andrew Hoffman
• Journal: Regenerative medicine (Regen Med) Vol. 11 Issue 1 Pg. 33-43 (Jan 2016) ISSN: 1746-076X [Electronic] England
• PMID: 26387424 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Cyclosporine

Topics

• Animals
• Crohn Disease (drug therapy, therapy)
• Cyclosporine (pharmacology, therapeutic use)
• Cytokines (blood)
• Disease Models, Animal
• Dogs
• Dose-Response Relationship, Drug
• Follow-Up Studies
• Heterografts
• Human Embryonic Stem Cells (cytology)
• Humans
• Immunomodulation
• Mesenchymal Stem Cell Transplantation
• Mesenchymal Stem Cells (cytology)
• Rectal Fistula (therapy)
• Stromal Cells (cytology)