Euphorbia supina inhibits inflammatory mediators in mouse bone marrow-derived mast cells and macrophages.

Abstract

Euphorbia supina has been traditionally used for the treatment of furuncle and bloody diarrhea relevant to the inflammatory process. It has been proven to have a variety of pharmacological efficacies including antiarthritic, detoxification, hemostatic, and diuretic activities. RAW 264.7 macrophages and bone marrow-derived mast cells (BMMCs) were used to determine the anti-inflammatory and anti-allergic effects of E. supina (ES). NO production was assayed by measuring the nitrite content of the supernatants of cultured RAW 264.7 cells. β-hexosaminidase, a marker of mast cell degranulation, was quantitated by spectrophotometric analysis. ELISA was used for the analysis of interleukin-6 expression, and Western blotting was used to analyze 5-LOX, iNOS, and MAPK activation. The relevant gene expression upon ES treatment was measured by RT-PCR. ES inhibited inducible nitric oxide synthase (iNOS) in RAW 264.7 cells, and IL-6 and LTC4 production in PMA- and A23187-induced BMMCs along with the downregulation of 5-LOX gene expression. Furthermore, in the present study, a decrease in p-ERK, p-JNK, and p-P38 expression, as well as the suppression of degranulation, were observed by treatment with ES. Further in vivo study revealed that ES treatment also remarkably inhibited xylene-induced mouse ear edema and MPO levels in mice ears. This study demonstrates that ES has a potential regulatory effect on the expression of inflammatory mediators through the inhibition of both the phosphorylation of MAPK signaling and the activation of degranulation.

• Authors: Hee-Sung Chae, Hyuk-Hwan Song, Young-Mi Kim, Hyeong-Kyu Lee, Sei-Ryang Oh, Young-Won Chin
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 29 Issue 2 Pg. 966-973 (Dec 2015) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 26386544 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Inflammation Mediators
• Interleukin-6
• interleukin-6, mouse
• Nitric Oxide
• Nitric Oxide Synthase Type II

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Bone Marrow Cells (drug effects)
• Cell Degranulation (drug effects)
• Edema (prevention & control)
• Euphorbia (chemistry)
• Inflammation Mediators (antagonists & inhibitors)
• Interleukin-6 (biosynthesis)
• Macrophages (drug effects)
• Mast Cells (drug effects)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred ICR
• Nitric Oxide (biosynthesis)
• Nitric Oxide Synthase Type II (antagonists & inhibitors)
• RAW 264.7 Cells
• Signal Transduction (drug effects)