Abstract | BACKGROUND: METHODS: Chronic constructive injury (CCI) was performed under anesthesia, on one side leg exposed by making a skin incision, and chromic gut ligatures were tied loosely around the sciatic nerve at 1 mm intervals. The treatment with grape seed proanthocyanidin extract (GSPE) (100 and 200 mg/kg, p.o.) was initiated on 7th day post-surgery and continued for next 14 days. Morphine (10 mg/kg, s.c.) alone and morphine in combination with GSPE (100 mg/kg, p.o.) were administered in CCI rats for 5 days starting from 7th day. On 3rd, 7th, 14th and 21st day, behavioral parameters ( mechanical allodynia and thermal hyperalgesia) were assessed. Then the animals were killed on 22nd day and biochemical parameters [ reduced glutathione (GSH), lipid peroxidation (LPO), catalase, nitrite, superoxide dismutase (SOD)] were assessed. RESULTS:
Ligation of the sciatic nerve significantly induced mechanical allodynia and thermal hyperalgesia and induces oxidative stress (increase in LPO and nitrite) and decline of anti-oxidant enzyme levels ( catalase, SOD, GSH) in sciatic nerve homogenate. GSPE (100 and 200 mg/kg, p.o.) attenuated all the behavioural and biochemical parameters. Morphine also significantly reversed the symptoms of neuropathic pain but produced tolerance after 5 days. Further, co-treatment of GSPE (100 mg/kg) with morphine (10 mg/kg, s.c.) in CCI rats significantly reversed the morphine tolerance and enhanced its anti-hyperalgesic effect as compared to the morphine-alone-treated group. CONCLUSIONS: In the present set of experiments, GSPE showed a significant anti-hyperalgesic and anti-nociceptive effect in rats.
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Authors | Gurmanpreet Kaur, Onkar Bedi, Nidhika Sharma, Shamsher Singh, Rahul Deshmukh, Puneet Kumar |
Journal | Journal of basic and clinical physiology and pharmacology
(J Basic Clin Physiol Pharmacol)
Vol. 27
Issue 1
Pg. 9-17
(Jan 2016)
ISSN: 2191-0286 [Electronic] Germany |
PMID | 26378488
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Analgesics
- Grape Seed Extract
- Grape Seed Proanthocyanidins
- Proanthocyanidins
- Morphine
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Topics |
- Analgesics
(administration & dosage, pharmacology)
- Animals
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Female
- Grape Seed Extract
(administration & dosage, pharmacology)
- Hyperalgesia
(drug therapy)
- Male
- Morphine
(pharmacology)
- Neuralgia
(drug therapy)
- Oxidative Stress
(drug effects)
- Proanthocyanidins
(administration & dosage, pharmacology)
- Rats
- Rats, Wistar
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