Abstract | BACKGROUND: OBJECTIVE: METHODS: Thirty-five Sprague-Dawley rats were allocated to a sham operation control group (SC group, n=10) or underwent surgery and MCAO (n=25). Postoperatively the latter group was randomly subdivided into EA or untreated (IR) groups. Cognitive impairment was assessed using the Morris water maze (MWM). Apoptosis was examined by detection of Bcl-2 and Bax expression in the cerebral cortex. RESULTS: The EA group had significantly decreased neurological deficit scores compared to the IR group (p<0.05). In the MWM test, significant differences in escape latency and route were observed between the EA and IR groups (p<0.05). Rats in the EA group performed better in the probe trial than those in the IR group (p<0.05). EA treatment markedly reduced the number of TUNEL-positive cells compared to the IR group (20.13±4.30% vs 38.40±3.38%; p<0.001). Reverse transcription-polymerase chain reaction (RT-PCR) results showed the Bcl-2/Bax ratio was significantly increased in the EA group compared to the IR group (1.61±0.19 vs 0.50±0.05, p<0.01). CONCLUSIONS: These findings suggest that EA ameliorates cognitive impairment of rats with IR injury by modulating Bcl-2 and Bax expression.
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Authors | Fang Liu, Yi-Jing Jiang, Hong-Jia Zhao, Li-Qun Yao, Li-Dian Chen |
Journal | Acupuncture in medicine : journal of the British Medical Acupuncture Society
(Acupunct Med)
Vol. 33
Issue 6
Pg. 478-84
(Dec 2015)
ISSN: 1759-9873 [Electronic] England |
PMID | 26376847
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/ |
Chemical References |
- Bax protein, rat
- bcl-2-Associated X Protein
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Topics |
- Acupuncture Points
- Animals
- Apoptosis
(genetics)
- Brain Ischemia
(complications, therapy)
- Cognition Disorders
(etiology, therapy)
- Disease Models, Animal
- Electroacupuncture
- Gene Expression Regulation
- Genes, bcl-2
- Neurons
(pathology)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(complications, therapy)
- bcl-2-Associated X Protein
(genetics)
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