Abstract |
DIA-2 is a herbal mixture containing standardized extract of Allium sativum and Lagerstroemia speciosa. Recently we have reported the anti-diabetic effect of DIA-2 in high fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic (T2D) rats. The purpose of this study was to investigate and compare the effects of DIA-2 with Rosiglitazone (RG) on plasma biomarkers of hepatocellular injury, liver carbohydrate metabolizing enzymes, glycogen content, oxidant/ antioxidant status and histopathological changes in T2D rats. ALT and ALP levels were significantly decreased after DIA-2 and RG treatment compared to T2D rats. Total protein and albumin remained unaltered in all the groups. Significant decrease in AST levels were observed after DIA-2 (125 mg/kg) and RG treatment. Hepatic hexokinase activity was significantly increased after RG and DIA-2 treatment and fructose-1, 6-bisphosphatase activity were inversely correlated with hexokinase activity. Hepatic gucose-6-phosphatase activity was significantly (p < 0.05) reduced after DIA-2 (62.5 mg/kg) and RG treatment. Lipid peroxides levels was significantly decreased in the liver of DIA-2 (62.5; p < 0.01 & 125 mg/kg; p < 0.05) treated animals. Hepatic glycogen content (p < 0.05) and antioxidant enzymes [SOD (p < 0.01; 62.5 mg/kg); GPx and GSH (125 mg/kg; p < 0.01)] were significantly increased after DIA-2 treatment. RG treatment on hepatic glycogen, GPx (p < 0.01) and SOD, GSH (p < 0.05) levels were significant when compared to T2D rats. These biochemical parameters were also correlated with histopathological evaluation. The above findings revealed that administration of DIA-2 could ameliorate the biochemical and histopathological changes in liver of T2D rats indicating the protective role of DIA-2 against HFD/STZ induced diabetes. In addition, DIA-2 and RG treatment resulted in amelioration of hepatic steatosis in T2D rats.
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Authors | K S Kesavanarayanan, R J Priya, C Selvakkumar, S Kavimani, D Prathiba |
Journal | European review for medical and pharmacological sciences
(Eur Rev Med Pharmacol Sci)
Vol. 19
Issue 16
Pg. 3107-17
(Aug 2015)
ISSN: 2284-0729 [Electronic] Italy |
PMID | 26367735
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Hypoglycemic Agents
- Plant Extracts
- Thiazolidinediones
- Rosiglitazone
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Topics |
- Animals
- Antioxidants
(pharmacology)
- Diabetes Mellitus, Experimental
(blood, drug therapy, pathology)
- Diet, High-Fat
(adverse effects)
- Fatty Liver
(blood, drug therapy, pathology)
- Hyperglycemia
(blood, drug therapy, pathology)
- Hypoglycemic Agents
(pharmacology)
- Liver Diseases
(drug therapy, pathology)
- Plant Extracts
(pharmacology)
- Random Allocation
- Rats
- Rosiglitazone
- Thiazolidinediones
(pharmacology)
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