Rosmarinic Acid Attenuates Sodium Taurocholate-Induced Acute Pancreatitis in Rats by Inhibiting Nuclear Factor-κB Activation.

Abstract

Rosmarinic Acid (RA), a caffeic acid ester, has been shown to exert anti-inflammation, anti-oxidant and antiallergic effects. Our study aimed to investigate the effect of RA in sodium taurocholate ( NaTC )-induced acute pancreatitis, both in vivo and in vitro. In vivo, RA (50 mg/kg) was administered intraperitoneally 2 h before sodium taurocholate injection. Rats were sacrificed 12 h, 24 h or 48 h after sodium taurocholate injection. Pretreatment with RA significantly ameliorated pancreas histopathological changes, decreased amylase and lipase activities in serum, lowered myeloperoxidase activity in the pancreas, reduced systematic and pancreatic interleukin-1 β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) levels, and inhibited NF-κB translocation in pancreas. In vitro, pretreating the fresh rat pancreatic acinar cells with 80 μ mol/L RA 2 h before 3750 nmol/L sodium taurocholate or 10 ng/L TNF-α administration significantly attenuated the reduction of isolated pancreatic acinar cell viability and inhibited the nuclear activation and translocation of NF-κB. Based on our findings, RA appears to attenuate damage in sodium taurocholate-induced acute pancreatitis and reduce the release of inflammatory cytokines by inhibiting the activation of NF-κB. These findings might provide a basis for investigating the therapeutic role of RA in managing acute pancreatits.

• Authors: Yu-Ting Fan, Guo-Jian Yin, Wen-Qin Xiao, Lei Qiu, Ge Yu, Yan-Ling Hu, Miao Xing, De-Qing Wu, Xiao-Feng Cang, Rong Wan, Xing-Peng Wang, Guo-Yong Hu
• Journal: The American journal of Chinese medicine (Am J Chin Med) Vol. 43 Issue 6 Pg. 1117-35 ( 2015) ISSN: 1793-6853 [Electronic] Singapore
• PMID: 26364660 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cinnamates
• Depsides
• Interleukin-1beta
• Interleukin-6
• NF-kappa B
• Plant Extracts
• Tumor Necrosis Factor-alpha
• Taurocholic Acid
• rosmarinic acid

Topics

• Animals
• Cinnamates (administration & dosage)
• Depsides (administration & dosage)
• Humans
• Interleukin-1beta (genetics, immunology)
• Interleukin-6 (genetics, immunology)
• Male
• NF-kappa B (genetics, immunology)
• Pancreas (drug effects, immunology)
• Pancreatitis (chemically induced, drug therapy, genetics, immunology)
• Plant Extracts (administration & dosage)
• Rats
• Rats, Sprague-Dawley
• Taurocholic Acid (adverse effects)
• Tumor Necrosis Factor-alpha (genetics, immunology)