The protective effects of tadalafil on renal damage following ischemia reperfusion injury in rats.

Abstract

Ischemia-reperfusion injury can cause renal damage, and phosphodiesterase inhibitors are reported to regulate antioxidant activity. We investigated the prevention of renal damage using tadalafil after renal ischemia reperfusion (I/R) injury in rats. A total of 21 adult male Wistar albino rats were randomly divided into three groups of seven, including Group 1-control, Group 2-I/R, and Group 3-tadalafil + I/R group (I/R-T group) received tadalafil intraperitoneally at 30 minutes before ischemia. Inducible nitric oxide synthase, endothelial nitric oxide synthase, malondialdehyde, and total antioxidant capacity levels were evaluated, and histopathological changes and apoptosis in the groups were examined. Tadalafil decreased malondialdehyde levels in the I/R group and increased the total antioxidant capacity level. Histopathological and immunohistochemical findings revealed that tadalafil decreased renal injury scores and the ratios of injured cells, as measured through apoptotic protease activating factor 1, inducible nitric oxide synthase, and endothelial nitric oxide synthase levels. We suggest that tadalafil has protective effects against I/R-related renal tissue injury.

• Authors: Bulent Erol, Tugrul Turker, Adem Tok, Sibel Bektas, Gorkem Mungan, Seyma Ozkanli, Bugra Karakas, Husnu Tokgoz, Bulent Akduman, Aydin Mungan
• Journal: The Kaohsiung journal of medical sciences (Kaohsiung J Med Sci) Vol. 31 Issue 9 Pg. 454-62 (Sep 2015) ISSN: 2410-8650 [Electronic] China (Republic : 1949- )
• PMID: 26362957 (Publication Type: Journal Article)
• Copyright: Copyright © 2015. Published by Elsevier Taiwan.

Chemical References

• Antioxidants
• Apaf1 protein, rat
• Apoptotic Protease-Activating Factor 1
• Protective Agents
• Malondialdehyde
• Tadalafil
• Nitric Oxide Synthase Type II
• Nitric Oxide Synthase Type III

Topics

• Animals
• Antioxidants (metabolism)
• Apoptotic Protease-Activating Factor 1 (metabolism)
• Immunohistochemistry
• Kidney (drug effects, pathology)
• Kidney Tubules (drug effects, enzymology, pathology)
• Malondialdehyde (blood)
• Nitric Oxide Synthase Type II (metabolism)
• Nitric Oxide Synthase Type III (metabolism)
• Protective Agents (pharmacology, therapeutic use)
• Rats, Wistar
• Reperfusion Injury (blood, drug therapy, pathology)
• Tadalafil