Real-time vascular imaging and photodynamic therapy efficacy with micelle-nanocarrier delivery of chlorin e6 to the microenvironment of melanoma.

Abstract

BACKGROUND:

Strategies combining anti-vascular therapy and vascular imaging may facilitate the prediction of early response and outcome in cancer treatment.

OBJECTIVE:

The aim of this study was to investigate the relationship between the tumor-associated vasculature in melanoma and to develop an approach for melanoma treatment by utilizing the free form and micelle form of the photosensitizer (PS) chlorin e6 in photodynamic therapy (PDT).

METHODS:

Green fluorescence protein (GFP) expressing B16-F10 melanoma cells were implanted into the mouse ear dermis. Ce6 in free form or in micelle form was administered via the tail vein. An OV100 imaging system was used to record the red fluorescence of Ce6 to obtain real-time vascular images in the GFP tumor.

RESULTS:

Compared to free Ce6, Ce6 linked to the micelle-nanocarrier depicted a much clearer vascular image and had an effective vascular destruction by PDT. Micelle Ce6 was localized in lysosomes and in the endoplasmic reticulum of cultured endothelial cells, implying an active endocytosis of the nano-carrier.

CONCLUSION:

Micelle Ce6 can serve as a bifunctional PS for vascular imaging and PDT, which facilitates its delivery in the tumor microenvironment.

• Authors: Chien-Hsing Lee, Ping-Shan Lai, Yen-Pei Lu, Hsuan-Ying Chen, Chee-Yin Chai, Rong-Kung Tsai, Kang-Tang Fang, Ming-Hsien Tsai, Chia-Yen Hsu, Chun-Cheng Hung, Deng-Chyang Wu, Hsin-Su Yu, Chung-Hsing Chang, Din-Ping Tsai
• Journal: Journal of dermatological science (J Dermatol Sci) Vol. 80 Issue 2 Pg. 124-32 (Nov 2015) ISSN: 1873-569X [Electronic] Netherlands
• PMID: 26360010 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Chlorophyllides
• Drug Carriers
• Micelles
• Photosensitizing Agents
• Porphyrins
• Green Fluorescent Proteins
• phytochlorin

Topics

• Animals
• Cell Line, Tumor
• Chemistry, Pharmaceutical
• Chlorophyllides
• Drug Carriers
• Endocytosis
• Endoplasmic Reticulum (metabolism)
• Genes, Reporter
• Green Fluorescent Proteins (genetics, metabolism)
• Human Umbilical Vein Endothelial Cells (metabolism)
• Humans
• Lysosomes (metabolism)
• Male
• Melanoma, Experimental (blood supply, drug therapy, genetics, metabolism, pathology)
• Mice, Nude
• Micelles
• Nanoparticles
• Neovascularization, Pathologic
• Optical Imaging (methods)
• Photochemotherapy (methods)
• Photosensitizing Agents (administration & dosage, chemistry)
• Porphyrins (administration & dosage, chemistry)
• Skin Neoplasms (blood supply, drug therapy, genetics, metabolism, pathology)
• Time Factors
• Tissue Distribution
• Transfection
• Tumor Microenvironment