Interaction of HSP20 with a viral RdRp changes its sub-cellular localization and distribution pattern in plants.

Abstract

Small heat shock proteins (sHSPs) perform a fundamental role in protecting cells against a wide array of stresses but their biological function during viral infection remains unknown. Rice stripe virus (RSV) causes a severe disease of rice in Eastern Asia. OsHSP20 and its homologue (NbHSP20) were used as baits in yeast two-hybrid (YTH) assays to screen an RSV cDNA library and were found to interact with the viral RNA-dependent RNA polymerase (RdRp) of RSV. Interactions were confirmed by pull-down and BiFC assays. Further analysis showed that the N-terminus (residues 1-296) of the RdRp was crucial for the interaction between the HSP20s and viral RdRp and responsible for the alteration of the sub-cellular localization and distribution pattern of HSP20s in protoplasts of rice and epidermal cells of Nicotiana benthamiana. This is the first report that a plant virus or a viral protein alters the expression pattern or sub-cellular distribution of sHSPs.

• Authors: Jing Li, Cong-Ying Xiang, Jian Yang, Jian-Ping Chen, Heng-Mu Zhang
• Journal: Scientific reports (Sci Rep) Vol. 5 Pg. 14016 (Sep 11 2015) ISSN: 2045-2322 [Electronic] England
• PMID: 26359114 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HSP20 Heat-Shock Proteins
• Plant Proteins
• Viral Proteins
• RNA-Dependent RNA Polymerase

Topics

• Gene Expression Regulation, Plant
• HSP20 Heat-Shock Proteins (genetics, metabolism)
• Host-Pathogen Interactions
• Intracellular Space (metabolism)
• Oryza (metabolism, virology)
• Plant Diseases (genetics, virology)
• Plant Proteins (metabolism)
• Plants (genetics, metabolism, virology)
• Protein Binding
• Protein Interaction Domains and Motifs (genetics)
• Protein Transport
• RNA-Dependent RNA Polymerase (chemistry, metabolism)
• Viral Proteins (metabolism)