Correlation Analysis Between Expression Levels of Hepatic Growth Hormone Receptor, Janus Kinase 2, Insulin-Like Growth Factor-I Genes and Dwarfism Phenotype in Bama Minipig.
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| Abstract |
Animal growth and development are complex and sophisticated biological metabolic processes, in which genes plays an important role. In this paper, we employed real-time quantitative PCR (RT-qPCR) to analyze the expression levels of hepatic GHR, JAK2 and IGF-I genes in 1, 30, 180 day of Bama minipig and Landrace with attempt to verify the correlation between the expression of these growth-associated genes and the dwarfism phenotype of Bama minipig. The results showed that the expression levels of these 3 genes in Bama minipigs were down-regulated expressed from 1 day to 30 day, and which was up-regulated expressed in Landrace. The expression levels of the 3 genes on 1, 30, 180 day were prominently higher in Landrace than in Bama minipigs. The significant differences of the 3 genes expression levels on 1 day between this two breeds indicate that different expressions of these genes might occur before birth. It is speculated that the down-regulated expression of the 3 genes may have a close correlation with the dwarfism phenotype of Bama minipig. More investigations in depth of this study is under progress with the help of biochip nanotechnology.
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| Authors | Haowen Yang, Qinyang Jiang, Dan Wu, Ganqiu Lan, Jing Fan, Yafen Guo, Baojian Chen, Xiurong Yang, Hesheng Jiang |
| Journal | Journal of nanoscience and nanotechnology (J Nanosci Nanotechnol) Vol. 15 Issue 2 Pg. 1789-92 (Feb 2015) ISSN: 1533-4880 [Print] United States |
| PMID | 26353733 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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