Inhibition of RANKL-induced osteoclastogenesis through the suppression of the ERK signaling pathway by astragaloside IV and attenuation of titanium-particle-induced osteolysis.

Abstract

Astragaloside IV (AS-IV) is a natural plant extract that enhances osteoblast activity, and therefore, has the potential to treat osteoclast‑related diseases. Such diseases include osteoporosis, periodontal disease, rheumatoid arthritis and aseptic prosthesis loosening. However, data associating the effects of AS‑IV on osteoclasts are limited. The aim of the present study was to assess the effect of AS‑IV on osteoclasts in vitro and in vivo. The in vitro studies demonstrated that AS‑IV exerts potent inhibitory effects on the ligand of the receptor activator of nuclear factor‑κB‑induced osteoclastogenesis and revealed the mechanism of action of AS‑IV, which inhibited osteoclastogenesis by suppression of the extracellular signal‑regulated kinase signaling pathway. The in vivo studies proved that AS‑IV attenuated titanium particle‑induced osteolysis in a mouse calvarial model. Collectively, the findings of the study suggest that AS‑IV is a potential natural agent for the treatment of osteoclast-related diseases.

• Authors: Mingjun Li, Wengang Wang, Li Geng, Yanru Qin, Wenjie Dong, Xudong Zhang, An Qin, Mingzhi Zhang
• Journal: International journal of molecular medicine (Int J Mol Med) Vol. 36 Issue 5 Pg. 1335-44 (Nov 2015) ISSN: 1791-244X [Electronic] Greece
• PMID: 26324422 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ligands
• RANK Ligand
• Receptor Activator of Nuclear Factor-kappa B
• Saponins
• Tnfsf11 protein, mouse
• Triterpenes
• astragaloside A
• Titanium
• Extracellular Signal-Regulated MAP Kinases

Topics

• Animals
• Cell Line
• Disease Models, Animal
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Ligands
• MAP Kinase Signaling System (drug effects)
• Mice
• Mice, Inbred C57BL
• Osteoclasts (drug effects, metabolism)
• Osteogenesis (drug effects)
• Osteolysis (chemically induced, drug therapy, metabolism)
• RANK Ligand (metabolism)
• Receptor Activator of Nuclear Factor-kappa B (metabolism)
• Saponins (pharmacology)
• Titanium (pharmacology)
• Triterpenes (pharmacology)