Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: The purposes of this study were to analyze the chemical constituents of G. brasiliensis branches and leaves and to evaluate the potential of isolated compounds to act as inhibitors of both the oxidative burst of stimulated neutrophils and oxidative damage in human erythrocyte membranes to verify the antioxidant and anti-inflammatory effects of this plant. MATERIALS AND METHODS: Neutrophils were isolated from the blood of healthy donors by Ficoll-Paque density gradient centrifugation. Superoxide anion and total reactive oxygen species (ROS) produced by stimulated neutrophils were measured by WST-1 reduction and luminol-enhanced chemiluminescence assays, respectively. Radical-induced lipoperoxidation and hemolysis were performed using erythrocytes from the blood of healthy donors. Compounds were isolated from G. brasiliensis branches and leaves by HPLC microfractionation, and structure elucidation of the isolated compounds was performed based on NMR and HR-MS analyses. RESULTS: CONCLUSIONS: These findings indicate that the biflavonoids extracted from G. brasiliensis branches and leaves modulate oxidative stress via inhibition of NADPH oxidase and ROS production by stimulated human neutrophils. Furthermore, the biflavonoids exhibited potent inhibition of oxidant hemolysis and lipid peroxidation induced by AAPH in human erythrocytes. Therefore, these studies suggest the use of G. brasiliensis extract as an antioxidant and anti-inflammatory agent.
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Authors | Phanuel Saroni Arwa, Maria Luiza Zeraik, Valdecir Farias Ximenes, Luiz Marcos da Fonseca, Vanderlan da Silva Bolzani, Dulce Helena Siqueira Silva |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 174
Pg. 410-8
(Nov 04 2015)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 26320685
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Flavonoids
- Phenols
- Reactive Oxygen Species
- Superoxides
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Topics |
- Anti-Inflammatory Agents, Non-Steroidal
(pharmacology)
- Erythrocyte Membrane
(drug effects)
- Flavonoids
(isolation & purification, pharmacology)
- Garcinia
(chemistry)
- Hemolysis
(drug effects)
- Humans
- In Vitro Techniques
- Lipid Peroxidation
(drug effects)
- Neutrophils
(drug effects, metabolism)
- Oxidative Stress
(drug effects)
- Phenols
(isolation & purification, pharmacology)
- Plant Leaves
(chemistry)
- Reactive Oxygen Species
(metabolism)
- Respiratory Burst
(drug effects)
- Superoxides
(metabolism)
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