Effect of endurance training on seizure susceptibility, behavioral changes and neuronal damage after kainate-induced status epilepticus in spontaneously hypertensive rats.

Abstract

The therapeutic efficacy of regular physical exercises in an animal model of epilepsy and depression comorbidity has been confirmed previously. In the present study, we examined the effects of endurance training on susceptibility to kainate (KA)-induced status epilepticus (SE), behavioral changes and neuronal damage in spontaneously hypertensive rats (SHRs). Male SHRs were randomly divided into two groups. One group was exercised on a treadmill with submaximal loading for four weeks and the other group was sedentary. Immediately after the training period, SE was evoked in half of the sedentary and trained rats by KA, while the other half of the two groups received saline. Basal systolic (SP), diastolic (DP) and mean arterial pressure (MAP) of all rats were measured at the beginning and at the end of the training period. Anxiety, memory and depression-like behaviour were evaluated a month after SE. The release of 5-HT in the hippocampus was measured using a liquid scintillation method and neuronal damage was analyzed by hematoxylin and eosin staining. SP and MAP of exercised SHRs decreased in comparison with the initial values. The increased resistance of SHRs to KA-induced SE was accompanied by an elongated latent seizure-free period, improved object recognition memory and antidepressant effect after the training program. While the anticonvulsant and positive behavioral effects of endurance training were accompanied by an increase of 5-HT release in the hippocampus, it did not exert neuroprotective activity. Our results indicate that prior exercise is an effective means to attenuate KA-induced seizures and comorbid behavioral changes in a model of hypertension and epilepsy suggesting a potential influence of hippocampal 5-HT on a comorbid depression. However, this beneficial impact does not prevent the development of epilepsy and concomitant brain damage.

• Authors: J Tchekalarova, M Shishmanova, D Atanasova, M Stefanova, L Alova, N Lazarov, K Georgieva
• Journal: Brain research (Brain Res) Vol. 1625 Pg. 39-53 (Nov 02 2015) ISSN: 1872-6240 [Electronic] Netherlands
• PMID: 26319691 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Excitatory Amino Acid Agonists
• Serotonin
• Kainic Acid

Topics

• Animals
• Behavior, Animal (drug effects)
• Blood Pressure (drug effects)
• Dark Adaptation (drug effects, physiology)
• Disease Models, Animal
• Disease Susceptibility
• Excitatory Amino Acid Agonists (toxicity)
• Exercise Test
• Exploratory Behavior (drug effects)
• Food Preferences (drug effects)
• Kainic Acid (toxicity)
• Male
• Maze Learning (drug effects)
• Mood Disorders (etiology, rehabilitation)
• Neurons (drug effects, pathology)
• Physical Endurance (physiology)
• Rats
• Rats, Inbred SHR
• Recognition, Psychology (drug effects)
• Serotonin (metabolism)
• Status Epilepticus (chemically induced, complications, pathology)