Acoustophoretic removal of proteins from blood components.

Abstract

This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97%, compared to just above 90% of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.

• Authors: Maria Tenje, Maria N Lundgren, Ann-Margret Swärd-Nilsson, Jens Kjeldsen-Kragh, Lena Lyxe, Andreas Lenshof
• Journal: Biomedical microdevices (Biomed Microdevices) Vol. 17 Issue 5 Pg. 95 (Oct 2015) ISSN: 1572-8781 [Electronic] United States
• PMID: 26315975 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Proteins

Topics

• Acoustics (instrumentation)
• Blood Component Removal (instrumentation)
• Blood Proteins (isolation & purification)
• Electrophoresis (instrumentation)
• Equipment Design
• Equipment Failure Analysis
• Erythrocytes (chemistry, radiation effects)
• High-Energy Shock Waves
• Humans
• Lab-On-A-Chip Devices
• Miniaturization
• Sonication (instrumentation)