Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.

Abstract

Enterovirus 71 (EV71), a member of Picornaviridae, causes severe neurological and systemic illness in children. To better understand the virus-host cell interactions, we performed a triple-SILAC-based quantitative proteomics study monitoring host cell proteome changes after EV71 infection. Based on the quantitative data for more than 4100 proteins, ∼17% of the proteins were found as significantly changed (p<0.01) at either 8 or 20 hours post infection. Five biological processes and seven protein classes showed significant differences. Functional screening of nine regulated proteins discovered the regulatory role of CHCH2, a mitochondrial protein known as a transcriptional activator for cytochrome c oxidase, in EV71 replication. Further studies showed that CHCH2 served as a negative regulator of innate immune responses. All MS data have been deposited in the ProteomeXchange with identifier PXD002483 (http://proteomecentral.proteomexchange.org/dataset/PXD002483).

• Authors: Hao-Yu Li, Lei-Ke Zhang, Xiu-Juan Zhu, Jun Shang, Xi Chen, Ying Zhu, Lin Guo
• Journal: Proteomics (Proteomics) Vol. 15 Issue 21 Pg. 3629-43 (Nov 2015) ISSN: 1615-9861 [Electronic] Germany
• PMID: 26306425 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Proteome

Topics

• Cell Line
• Disease Progression
• Enterovirus A, Human (immunology, physiology)
• Enterovirus Infections (immunology, metabolism, pathology)
• Host-Pathogen Interactions
• Humans
• Immunity, Innate
• Proteome (analysis, immunology, metabolism)
• Proteomics (methods)